FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate1_75.3520000005ba67.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate1_75.3520000005ba67.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17822
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1290.7238244865896084No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1220.6845471888676916No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1060.5947705083604534No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA680.38155089215576254No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT670.37593984962406013No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA670.37593984962406013No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA590.33105150937044103No Hit
GTGAATGGGTCGTTGGTCAAAAAAAAAAAAAAA560.3142183817753339No Hit
GTGAATGGGTCGTTGGAAAAAAAAAAAAAAAAA540.30299629671192907No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA510.2861631691168219No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA460.25810795645831No Hit
GTGAATGGGTCGTTGGTAAAAAAAAAAAAAAAA440.24688587139490517No Hit
GAGTACGTTTCGACCAAAAAAAAAAAAAAAAAA440.24688587139490517No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA410.230052743799798No Hit
ACGCAGAGTGAATGGGTCGTTGGTCAAAAAAAA400.2244417012680956No Hit
GTTTCGACCAAAAAAAAAAAAAAAAAAAAAAAA390.21883065873639324No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA350.19638648860958366No Hit
GTGAATGGGATCGTTGGAAAAAAAAAAAAAAAA330.18516440354617889No Hit
GGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA330.18516440354617889No Hit
GTCGTTGGTCGAAACGTACTCTGCGTTGATACC320.1795533610144765No Hit
ACGCAGAGTGAATGGGTCGTTGGTAAAAAAAAA310.17394231848277408No Hit
GTGAATGGGGTCGTTGGAAAAAAAAAAAAAAAA280.15710919088766695No Hit
GTGAATGGGTCGTTGGTCGAAAAAAAAAAAAAA280.15710919088766695No Hit
GAGTGAATGGGGTCGTTGGAAAAAAAAAAAAAA270.15149814835596453No Hit
ACGCAGAGTGAATGGGATCGTTGGTCAAAAAAA260.14588710582426215No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA260.14588710582426215No Hit
TCGTTGGTCGAAACGTACTCTGCGTTGATACCA250.14027606329255976No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA250.14027606329255976No Hit
GAATGGGAAGCAGTGGTATCAAAAAAAAAAAAA240.13466502076085735No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT230.129053978229155No Hit
ACGCAGAGTGAATGGGGTCGTTGGAAAAAAAAA230.129053978229155No Hit
AACGCAGAGTGAATGGGTCGTTGGAAAAAAAAA230.129053978229155No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.129053978229155No Hit
ACGCAGAGTGAATGGGATCGTTGGAAAAAAAAA230.129053978229155No Hit
GAGTGAATGGGGTACGTTTCGACCAAAAAAAAA220.12344293569745259No Hit
GAGTGAATGGGATCGTTGGTCAAAAAAAAAAAA220.12344293569745259No Hit
GAGTGAATGGGATCGTTGGAAAAAAAAAAAAAA220.12344293569745259No Hit
CTCGTTGGTCGAAACGTACTCTGCGTTGATACC220.12344293569745259No Hit
ACGCAGAGTGAATGGGGTACGTTTCGACCAAAA220.12344293569745259No Hit
GGTACGTTTCGACCAAAAAAAAAAAAAAAAAAA210.1178318931657502No Hit
GTGAATGGGATCGTTGGTCAAAAAAAAAAAAAA210.1178318931657502No Hit
TATCAACGCAGAGTGAATGGGTCGTTGGTCAAA200.1122208506340478No Hit
CGTTTCGACCAAAAAAAAAAAAAAAAAAAAAAA190.10660980810234541No Hit
GAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAA190.10660980810234541No Hit
AACGCAGAGTGAATGGGATCGTTGGTCAAAAAA190.10660980810234541No Hit
ACGCAGAGTGAATGGGTCGTTGGAAAAAAAAAA180.10099876557064302No Hit
ATCGTTGGTCGAAACGTACTCTGCGTTGATACC180.10099876557064302No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCGT3300.014.72727327
CTTCGTT657.141969E-414.5384617
GCGTTGA852.2330265E-514.29411827
TATCAAC1551.1641532E-1013.9354851
TTCGTTG1358.6242653E-712.08
CTCTGCG4200.011.57142826
ACTCTGC4300.011.30232425
TGCGTTG1102.499404E-411.04545526
GTACTCT4700.010.34042623
TACTCTG4750.010.23157924
CGTACTC4800.010.12522
ATCAACG2007.912422E-810.1249992
ACGTACT4900.09.91836721
AATGGGC1100.00316466189.81818211
CAACGCA2254.125104E-89.64
CGAAACG4950.09.54545417
AACGTAC5000.09.4520
GAAACGT5000.09.4518
TCAACGC2152.2778113E-79.4186043
AAACGTA5050.09.35643619
Produced by FastQC (version 0.11.2)