FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate1_74.3520000005ba4e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate1_74.3520000005ba4e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21004
Sequences flagged as poor quality0
Sequence length33
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACACAGACAGTACGTACTCTGCGTTGATACCA5372.556655875071415No Hit
GTGAATGGGACACAGACAAAAAAAAAAAAAAAA3801.8091792039611505No Hit
GACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3001.4282993715482766No Hit
GTGAATGGGACACAGACAGAAAAAAAAAAAAAA2761.3140354218244144No Hit
AGACAGTACGTACTCTGCGTTGATACCACTGCT2371.1283565035231384No Hit
GACACAGACAGAAAAAAAAAAAAAAAAAAAAAA2291.0902685202818512No Hit
ACGCAGAGTGAATGGGACACAGACAAAAAAAAA2281.0855075223766901No Hit
GACACAGACAGTACAAAAAAAAAAAAAAAAAAA1690.8046086459721957No Hit
TATCAACGCAGAGTGAATGGGACACAGACAAAA1590.7569986669205866No Hit
ACGCAGAGTGAATGGGACACAGACAGAAAAAAA1430.6808227004380117No Hit
GAGTGAATGGGACACAGACAAAAAAAAAAAAAA1290.614168729765759No Hit
GAGTGAATGGGACACAGACAGAAAAAAAAAAAA1180.5617977528089888No Hit
AACGCAGAGTGAATGGGACACAGACAAAAAAAA1180.5617977528089888No Hit
GACACAGACAAAAAAAAAAAAAAAAAAAAAAAA1140.5427537611883451No Hit
GACACAGACAGTAAAAAAAAAAAAAAAAAAAAA1130.5379927632831841No Hit
TATCAACGCAGAGTGAATGGGACACAGACAGAA1040.4951437821367358No Hit
TATCAACGCAGAGTGAATGGGACACAGACAGTA1000.47609979051609214No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA980.4665777947057703No Hit
GAATGGGACACAGACAAAAAAAAAAAAAAAAAA930.4427728051799657No Hit
GACAGAAAAAAAAAAAAAAAAAAAAAAAAAAAA890.423728813559322No Hit
GTATCAACGCAGAGTGAATGGGACACAGACAAA800.3808798324128737No Hit
CAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA800.3808798324128737No Hit
GTGAATGGGACACAGACAGTAAAAAAAAAAAAA790.37611883450771283No Hit
GTGAATGGGGACACAGACAAAAAAAAAAAAAAA770.366596838697391No Hit
AACGCAGAGTGAATGGGACACAGACAGAAAAAA760.36183584079223No Hit
GTGAATGGGACACAGACAGTACAAAAAAAAAAA740.3523138449819082No Hit
GAATGGGGACACAGACAAAAAAAAAAAAAAAAA730.34755284707674733No Hit
GTGAATGGGGACACAGACAGAAAAAAAAAAAAA720.34279184917158634No Hit
GTGAATGGGACACAGAAAAAAAAAAAAAAAAAA720.34279184917158634No Hit
GTACGTACTCAAAAAAAAAAAAAAAAAAAAAAA710.33803085126642546No Hit
GAATGGGACACAGACAGAAAAAAAAAAAAAAAA700.3332698533612645No Hit
GACACAGACAGTACGAAAAAAAAAAAAAAAAAA670.3189868596457817No Hit
GACAGTACAAAAAAAAAAAAAAAAAAAAAAAAA610.2904208722148162No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA600.28565987430965534No Hit
GACAGTAAAAAAAAAAAAAAAAAAAAAAAAAAA570.27137688059417253No Hit
CAACTCAGAGTGAATGGGACACAGACAAAAAAA570.27137688059417253No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA550.2618548847838507No Hit
CAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAA530.25233288897352885No Hit
ACGCAGAGTGAATGGGACACAGACAGTAAAAAA510.242810893163207No Hit
GACAGTACGTACTCAAAAAAAAAAAAAAAAAAA500.23804989525804607No Hit
CAGACAAAAAAAAAAAAAAAAAAAAAAAAAAAA500.23804989525804607No Hit
GAGTGAATGGGGACACAGACAGAAAAAAAAAAA480.22852789944772423No Hit
GAGTGAATGGGGACACAGACAAAAAAAAAAAAA480.22852789944772423No Hit
ACAGTACGTACTCTGCGTTGATACCACTGCTTC470.22376690154256332No Hit
GAATGGGGACACAGACAGAAAAAAAAAAAAAAA460.21900590363740238No Hit
ATGGGACACAGACAAAAAAAAAAAAAAAAAAAA460.21900590363740238No Hit
GTATCATCGCAGAGTGAATGGGACACAGACAAA460.21900590363740238No Hit
ACACAGACAGTACGTACTCTGCGTTGATACCAC460.21900590363740238No Hit
CAACTCAGAGTGAATGGGACACAGACAGAAAAA460.21900590363740238No Hit
GTATCAACGCAGAGTGAATGGGACACAGACAGA450.21424490573224148No Hit
GTGAATGGGGACACAGAAAAAAAAAAAAAAAAA450.21424490573224148No Hit
ACGCAGAGTGAATGGGACACAGAAAAAAAAAAA440.20948390782708054No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA430.20472290992191966No Hit
GACACAGACAGTACGTAAAAAAAAAAAAAAAAA420.1999619120167587No Hit
GGACACAGACAGTACGTACTCTGCGTTGATACC410.1952009141115978No Hit
GTATCAACGCAGAGTGAATGGGACACAGACAGT400.19043991620643685No Hit
GACACAGACAGTACGTACTCAAAAAAAAAAAAA400.19043991620643685No Hit
ATGGGACACAGACAGAAAAAAAAAAAAAAAAAA400.19043991620643685No Hit
ATCAACGCAGAGTGAATGGGACACAGACAAAAA400.19043991620643685No Hit
GAGTGAATGGGACACAGACAGTAAAAAAAAAAA390.18567891830127595No Hit
TATCAACGCAGAGTGAATGGGACACAGAAAAAA380.180917920396115No Hit
AATGGGACACAGACAAAAAAAAAAAAAAAAAAA380.180917920396115No Hit
ATCAACGCAGAGTGAATGGGACACAGACAGAAA370.1761569224909541No Hit
GTGAATGGGACACAAAAAAAAAAAAAAAAAAAA370.1761569224909541No Hit
ACGCAGAGTGAATGGGACACAGACAGTACAAAA360.17139592458579317No Hit
CAACGCAGAGTGAATGGGACACAGACAAAAAAA350.16663492668063226No Hit
AGACAGTACAAAAAAAAAAAAAAAAAAAAAAAA340.16187392877547135No Hit
AATGGGACACAGACAGAAAAAAAAAAAAAAAAA340.16187392877547135No Hit
GGACACAGACAGAAAAAAAAAAAAAAAAAAAAA340.16187392877547135No Hit
CAGACAGAAAAAAAAAAAAAAAAAAAAAAAAAA330.15711293087031042No Hit
ACGCAGAGTGAATGGGACACAAAAAAAAAAAAA330.15711293087031042No Hit
GAGTGAATGGGACACAGACAGTACAAAAAAAAA330.15711293087031042No Hit
ACGCAGAGTGAATGGGGACACAGACAGAAAAAA320.1523519329651495No Hit
CAGTACGTACTCAAAAAAAAAAAAAAAAAAAAA310.14759093505998858No Hit
GTATCATCGCAGAGTGAATGGGACACAGACAGA310.14759093505998858No Hit
CAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA300.14282993715482767No Hit
GTACGTAAAAAAAAAAAAAAAAAAAAAAAAAAA300.14282993715482767No Hit
GGTATCAACGCAGAGTGAATGGGACACAGACAG300.14282993715482767No Hit
TATCTACGCAGAGTGAATGGGACACAGACAAAA290.13806893924966673No Hit
GAATGGGACACAGACAGTAAAAAAAAAAAAAAA290.13806893924966673No Hit
ACGCAGAGTGAATGGGGACACAGACAAAAAAAA280.13330794134450583No Hit
AACGTAGAGTGAATGGGACACAGACAAAAAAAA270.1285469434393449No Hit
AACGCAGAGTGAATGGGACACAGACAGTAAAAA270.1285469434393449No Hit
GGACACAGACAAAAAAAAAAAAAAAAAAAAAAA270.1285469434393449No Hit
GAGTGAATGGGACACAGAAAAAAAAAAAAAAAA270.1285469434393449No Hit
GAATGGGACACAGAAAAAAAAAAAAAAAAAAAA260.12378594553418396No Hit
GTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.11902494762902303No Hit
TATCTACGCAGAGTGAATGGGACACAGACAGAA250.11902494762902303No Hit
ACGCAGAGTGAATGGGACACAGACAGTACGAAA240.11426394972386211No Hit
GGCACAGACAGAAAAAAAAAAAAAAAAAAAAAA240.11426394972386211No Hit
GTATCATCGCAGAGTGAATGGGACACAGACAGT240.11426394972386211No Hit
AACGCAGAGTGAATGGGACACAGACAGTACAAA240.11426394972386211No Hit
TATCAACGCAGAGTGAATGGGACACAAAAAAAA230.10950295181870119No Hit
ACGCTGAGTGAATGGGACACAGACAAAAAAAAA230.10950295181870119No Hit
AACGCAGAGTGAATGGGACACAGAAAAAAAAAA230.10950295181870119No Hit
CAACTCAGAGTGAATGGGGACACAGACAAAAAA230.10950295181870119No Hit
ACGCAGAGTGAATGGGACACAGACAGTACGTAC220.10474195391354027No Hit
TATCAACGCAGAGTGAATGGGGACACAGACAAA220.10474195391354027No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTGCT356.1062037E-627.04545227
ACCACTG356.2216423E-626.98090725
GTATCAT306.867305E-526.9809071
CCACTGC356.2216423E-626.98090726
TATCATC306.867305E-526.9809072
TACCACT401.7208427E-523.66477224
CAACTCA351.9570604E-423.1264921
ATCATCG351.9570604E-423.1264923
TCATCGC351.9570604E-423.1264924
CATCGCA351.9570604E-423.1264925
TAAATGG300.002200830322.4840894
AGTAAAT300.002200830322.4840892
GTAAATG300.002200830322.4840893
TCGCAGA404.827746E-420.235687
ATCGCAG404.827746E-420.235686
GAGTAAA350.005379228419.2720781
AAATGGG350.005379228419.2720785
AACTCAG450.001066632717.987272
TCAGAGT450.001066632717.987275
CTCAGAG450.001066632717.987274
Produced by FastQC (version 0.11.2)