FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate1_71.3520000005b9ef.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate1_71.3520000005b9ef.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21544
Sequences flagged as poor quality0
Sequence length33
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGATACGTACTCAAAAAAAAAAAAAAAAAAA5092.362606758262161No Hit
GATACGTACTCAAAAAAAAAAAAAAAAAAAAAA3121.4481990345339768No Hit
GTGAATGGGATAGCAGATACGTACAAAAAAAAA2030.9422577051615298No Hit
TATCAACGCAGAGTGAATGGGATAGCAGATACG1990.9236910508726328No Hit
GTGAATGGGATAGCAGATACGTACTCAAAAAAA1850.8587077608614928No Hit
GCAGATACGTACAAAAAAAAAAAAAAAAAAAAA1740.8076494615670257No Hit
GGATAGCAGATACGTACTCTGCGTTGATACCAC1720.7983661344225771No Hit
GTACTCAAAAAAAAAAAAAAAAAAAAAAAAAAA1310.6080579279613814No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA970.4502413665057557No Hit
GATACGTACAAAAAAAAAAAAAAAAAAAAAAAA970.4502413665057557No Hit
GGATAGCAGATACGTACAAAAAAAAAAAAAAAA940.4363163757890828No Hit
ACGCAGAGTGAATGGGATAGCAGATACGTACAA880.40846639435573706No Hit
ACGCAGAGTGAATGGGATAGCAGATACGTACTC850.39454140363906426No Hit
GCAGATACGTACTAAAAAAAAAAAAAAAAAAAA820.3806164129223914No Hit
GGATAGCAGATACGTACTCAAAAAAAAAAAAAA770.35740809506126997No Hit
GTATCAACGCAGAGTGAATGGGATAGCAGATAC770.35740809506126997No Hit
GATACGTACTAAAAAAAAAAAAAAAAAAAAAAA710.32955811362792425No Hit
TACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA700.32491645005569997No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA660.3063497957668028No Hit
GATAGCAGATACGTACTCTGCGTTGATACCACT650.30170813219457854No Hit
TATCAACGCAGAGTGAATGGGTAGCAGATACGT630.29242480505012997No Hit
GAGTGAATGGGATAGCAGATACGTACTCAAAAA610.2831414779056814No Hit
ATCAACGCAGAGTGAATGGGATAGCAGATACGT570.26457482361678425No Hit
AACGCAGAGTGAATGGGATAGCAGATACGTACT560.25993316004455996No Hit
GTGAATGGGATAGCAGATACGTACTAAAAAAAA520.24136650575566285No Hit
CGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAA510.23672484218343853No Hit
CAGATACGTACTCAAAAAAAAAAAAAAAAAAAA500.23208317861121425No Hit
CGTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA480.22279985146676567No Hit
GTGAATGGGCGGATAGCAGAAAAAAAAAAAAAA470.2181581878945414No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA470.2181581878945414No Hit
GTGAATGGGATAGCAGAAAAAAAAAAAAAAAAA460.21351652432231713No Hit
GAGTGAATGGGATAGCAGATACGTACAAAAAAA460.21351652432231713No Hit
GTACTAAAAAAAAAAAAAAAAAAAAAAAAAAAA450.20887486075009284No Hit
GTGAATGGGATAGCAGATACGTAAAAAAAAAAA430.19959153360564427No Hit
AACGCAGAGTGAATGGGATAGCAGATACGTACA410.1903082064611957No Hit
GTATCATCGCAGAGTGAATGGGATAGCAGATAC390.1810248793167471No Hit
TAGCAGATACGTACAAAAAAAAAAAAAAAAAAA380.17638321574452284No Hit
GGTATCAACGCAGAGTGAATGGGATAGCAGATA380.17638321574452284No Hit
CAACGCAGAGTGAATGGGATAGCAGATACGTAC370.17174155217229856No Hit
ACGCAGAGTGAATGGGTAGCAGATACGTACTCA350.16245822502784998No Hit
CAACTCAGAGTGAATGGGATAGCAGATACGTAC350.16245822502784998No Hit
ACGCAGAGTGAATGGGCGGATAGCAGAAAAAAA350.16245822502784998No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA340.1578165614556257No Hit
ACGCAGAGTGAATGGGACGGATAGCAGAAAAAA330.1531748978834014No Hit
TATCTACGCAGAGTGAATGGGATAGCAGATACG330.1531748978834014No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA310.14389157073895284No Hit
GTATCAACGCAGAGTGAATGGGTAGCAGATACG310.14389157073895284No Hit
GAATGGGATAGCAGATACGTACTCAAAAAAAAA310.14389157073895284No Hit
GTGAATGGGACGGATAGCAGAAAAAAAAAAAAA300.13924990716672855No Hit
AACGCAGAGTGAATGGGTAGCAGATACGTACTC290.13460824359450427No Hit
GAGTGAATGGGACGGATAGCAGAAAAAAAAAAA280.12996658002227998No Hit
GAATGGGATAGCAGATACGTACAAAAAAAAAAA280.12996658002227998No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA280.12996658002227998No Hit
GCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.12996658002227998No Hit
GCGGATAGCAGATACGTACTCTGCGTTGATACC270.1253249164500557No Hit
TATCAACGCAGAGTGAATGGGACGGATAGCAGA270.1253249164500557No Hit
GGATAGCAGAAAAAAAAAAAAAAAAAAAAAAAA270.1253249164500557No Hit
GGATAGCAGATACGTACTAAAAAAAAAAAAAAA260.12068325287783142No Hit
CCGGATAGCAGATACGTACTCTGCGTTGATACC260.12068325287783142No Hit
GGATAGCAGATACGTACTCTGCGTTTATACCAC260.12068325287783142No Hit
GTGAATGGGTAGCAGATACGTACTCAAAAAAAA260.12068325287783142No Hit
GATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.11604158930560712No Hit
ACGCAGAGTGAATGGGATAGCAGATACGTACTA250.11604158930560712No Hit
ACGCAGAGTGAATGGGTAGCAGATACGTACAAA250.11604158930560712No Hit
TATCAACGCAGAGTGAATGGGCGGATAGCAGAA250.11604158930560712No Hit
GCAGATACGTAAAAAAAAAAAAAAAAAAAAAAA250.11604158930560712No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA250.11604158930560712No Hit
ATCAACGCAGAGTGAATGGGTAGCAGATACGTA240.11139992573338284No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA240.11139992573338284No Hit
ACGCAGAGTGAATGGGCGGATAGCAAAAAAAAA240.11139992573338284No Hit
TAGCAGATACGTACTCAAAAAAAAAAAAAAAAA240.11139992573338284No Hit
GATACGTACTCTAAAAAAAAAAAAAAAAAAAAA230.10675826216115857No Hit
GAGTGAATGGGATAGCAGATACGTACTAAAAAA230.10675826216115857No Hit
AACGCAGAGTGAATGGGTAGCAGATACGTACAA230.10675826216115857No Hit
GTGAATGGGCGGATAGCAAAAAAAAAAAAAAAA230.10675826216115857No Hit
GAGTGAATGGGTAGCAGATACGTACTCAAAAAA220.10211659858893427No Hit
GTGAATGGGTAGCAGATACGTACAAAAAAAAAA220.10211659858893427No Hit
GCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.10211659858893427No Hit
AACGCAGAGTGAATGGGCGGATAGCAGAAAAAA220.10211659858893427No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGC200.00842707127.03
CTCAGAG356.197548E-627.04
TCAGAGT401.7464605E-523.6255
CAACTCA351.950039E-423.1428591
AACTCAG351.950039E-423.1428592
ACTCAGA351.950039E-423.1428593
TCGCAGA351.950039E-423.1428595
ATCATCG351.950039E-423.1428591
ATCGCAG351.950039E-423.1428594
TCATCGC351.950039E-423.1428592
CATCGCA351.950039E-423.1428593
TGGTATC552.0259095E-417.1818182
GTGGTAT603.935061E-415.7500011
TATCAAA657.2289695E-414.5384625
TATCAAC2000.014.1749991
ATACCAC802.221051E-413.527
ATCAAAA700.001266446813.56
TCTGCGT3300.012.68181827
GGTATCA750.002129760812.5999993
TCAACGC2150.012.558143
Produced by FastQC (version 0.11.2)