FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate1_70.3520000005b9c5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate1_70.3520000005b9c5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21665
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAATGGGTAGTTCTGACACAAAAAAAAAAAA3101.4308792984075698No Hit
GTTCTGACACGTACAAAAAAAAAAAAAAAAAAA2301.0616201246249712No Hit
TATCAACGCAGAGTGAATGGGTAGTTCTGACAC2291.0570043849526887No Hit
ACGCAGAGTGAATGGGTAGTTCTGACACAAAAA1980.9139164551119316No Hit
CGGTAGTTCTGACACGTACTCTGCGTTGATACC1470.678513731825525No Hit
GTATCAACGCAGAGTGAATGGGTAGTTCTGACA1210.5585045003461805No Hit
GGGTAGTTCTGACACAAAAAAAAAAAAAAAAAA1180.544657281329333No Hit
GTGAATGGGGTAGTTCTGACACAAAAAAAAAAA1170.5400415416570505No Hit
GTTCTGACACAAAAAAAAAAAAAAAAAAAAAAA1140.526194322640203No Hit
GTGAATGGGGTAGTTCAAAAAAAAAAAAAAAAA1080.4984998846065082No Hit
GTTCTGACACGTACTCAAAAAAAAAAAAAAAAA1070.4938841449342257No Hit
GGGTAGTTCTGACACGTACTCTGCGTTGATACC990.4569582275559658No Hit
GTGAATGGGTAGTTCAAAAAAAAAAAAAAAAAA940.4338795291945534No Hit
AACGCAGAGTGAATGGGTAGTTCTGACACAAAA900.4154165705054235No Hit
GAGTGAATGGGTAGTTCTGACACAAAAAAAAAA770.3554119547657512No Hit
GTGAATGGGTAGTTCTGACACGAAAAAAAAAAA750.34618047542118624No Hit
TTCTGACACAAAAAAAAAAAAAAAAAAAAAAAA730.3369489960766213No Hit
CGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAA730.3369489960766213No Hit
TATCAACGCAGAGTGAATGGGGTAGTTCTGACA670.30925455804292634No Hit
GTTCTGACACGTAAAAAAAAAAAAAAAAAAAAA650.3000230786983614No Hit
GTGAATGGGTAGTTCTGACACGTACAAAAAAAA650.3000230786983614No Hit
ATCAACGCAGAGTGAATGGGTAGTTCTGACACA640.2954073390260789No Hit
GTTCTGACACGAAAAAAAAAAAAAAAAAAAAAA630.29079159935379645No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA600.27694438033694896No Hit
GTGAATGGGTAGTTCTGACAAAAAAAAAAAAAA500.23078698361412414No Hit
AGGTAGTTCTGACACGTACTCTGCGTTGATACC470.2169397645972767No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA460.21232402492499425No Hit
ACGCAGAGTGAATGGGTAGTTCTGACACGAAAA460.21232402492499425No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA460.21232402492499425No Hit
GAGTGAATGGGGTAGTTCTGACACAAAAAAAAA450.20770828525271176No Hit
AACGCAGAGTGAATGGGTAGTTCTGACACGTAC440.20309254558042925No Hit
GGTATCAACGCAGAGTGAATGGGTAGTTCTGAC440.20309254558042925No Hit
ACGCAGAGTGAATGGGGTAGTTCAAAAAAAAAA430.19847680590814676No Hit
GAGTGAATGGGGTAGTTCAAAAAAAAAAAAAAA420.1938610662358643No Hit
ACGCAGAGTGAATGGGTAGTTCAAAAAAAAAAA410.1892453265635818No Hit
GAATGGGTAGTTCTGACACAAAAAAAAAAAAAA410.1892453265635818No Hit
GTGAATGGGTAGTTCTGACACGTACTCAAAAAA410.1892453265635818No Hit
CACGTACAAAAAAAAAAAAAAAAAAAAAAAAAA410.1892453265635818No Hit
ACGCAGAGTGAATGGGTAGTTCTGACACGTACT410.1892453265635818No Hit
GGGTAGTTCTGACACGTACAAAAAAAAAAAAAA410.1892453265635818No Hit
GTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA400.18462958689129935No Hit
CAACGCAGAGTGAATGGGTAGTTCTGACACAAA390.18001384721901684No Hit
ACGCAGAGTGAATGGGGTAGTTCTGACACGTAC390.18001384721901684No Hit
GTGAATGGGTAGTTCTGAAAAAAAAAAAAAAAA380.17539810754673438No Hit
GTGAATGGGGTAGTTCTGACACGTACAAAAAAA380.17539810754673438No Hit
GTGAATGGGTAGTTCTGACACGTAAAAAAAAAA370.17078236787445186No Hit
ACGCAGAGTGAATGGGTAGTTCTGACACGTACA370.17078236787445186No Hit
GACACAAAAAAAAAAAAAAAAAAAAAAAAAAAA350.16155088852988692No Hit
GTATCAACGCAGAGTGAATGGGGTAGTTCTGAC350.16155088852988692No Hit
ACGCAGAGTGAATGGGGTAGTTCTGACACAAAA350.16155088852988692No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA350.16155088852988692No Hit
TATCAACGCAGAGTGAATGGGGTAGTTCAAAAA340.15693514885760443No Hit
TGGTAGTTCTGACACGTACTCTGCGTTGATACC340.15693514885760443No Hit
GACACGTACTCAAAAAAAAAAAAAAAAAAAAAA330.15231940918532194No Hit
AACGCAGAGTGAATGGGTAGTTCTGACACGAAA320.14770366951303945No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA310.143087929840757No Hit
ATCAACGCAGAGTGAATGGGTAGTTCTGACACG300.13847219016847448No Hit
CGTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA300.13847219016847448No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA300.13847219016847448No Hit
GTATCATCGCAGAGTGAATGGGTAGTTCTGACA300.13847219016847448No Hit
GTGAATGGGGTAGTTCTGACACGTACTCAAAAA290.13385645049619202No Hit
CACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.13385645049619202No Hit
GGGTAGTTCAAAAAAAAAAAAAAAAAAAAAAAA290.13385645049619202No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT280.12924071082390953No Hit
ATCAACGCAGAGTGAATGGGGTAGTTCTGACAC280.12924071082390953No Hit
GAATGGGGTAGTTCAAAAAAAAAAAAAAAAAAA270.12462497115162705No Hit
GAGTGAATGGGTAGTTCTGACACGAAAAAAAAA270.12462497115162705No Hit
ACGCAGAGTGAATGGGTAGTTCTGACAAAAAAA260.12000923147934457No Hit
CAACGCAGAGTGAATGGGTAGTTCTGACACGTA250.11539349180706207No Hit
GGGTAGTTCTGACACGAAAAAAAAAAAAAAAAA240.1107777521347796No Hit
GTAGTTCTGACACGTACTCTGCGTTGATACCAC240.1107777521347796No Hit
GTGAATGGGGTAGTTCTGACAAAAAAAAAAAAA240.1107777521347796No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA230.10616201246249712No Hit
TATCAACGCAGAGTGAATGGGTAGTTCTGACAA220.10154627279021462No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACC405.6306453E-727.027
GTTGATA451.5732094E-624.00000225
TTGATAC451.5732094E-624.00000226
AACGTAG350.005362991219.2857151
ACGTACA350.005362991219.2857159
GTATCAA1252.2439053E-814.041
CGTTGAT802.2221913E-413.524
TGCGTTG1901.6370905E-1112.78947427
TATCAAC1501.7435013E-812.5999991
ATCAACG1501.7435013E-812.5999992
ATGGGGT1707.55972E-911.9117655
TGGGGTA1707.55972E-911.9117656
TCAACGC1553.7318932E-711.3225813
CTGCGTT3200.010.9687527
GGGGTAG2408.54925E-1110.6874997
TCTGCGT5350.010.34579427
GCGTTGA1050.002183481410.28571423
AATGGGG3004.984031E-109.04
CAACGCA1957.110164E-69.04
CTCTGCG6100.08.85245926
Produced by FastQC (version 0.11.2)