FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate1_7.3520000005b246.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate1_7.3520000005b246.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60793
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTGAATGGGCATTGCTGAAGA3250.534601023144112No Hit
GCATTGCTGAAGACGTACTCTGCGTTGATACCA3000.49347786751764183No Hit
GTGAATGGGCATTGCTGAAGAAAAAAAAAAAAA2830.46551412169164214No Hit
GTGAATGGGCATTGCTGAAAAAAAAAAAAAAAA2790.45893441679140695No Hit
GCTGAAGACGTACTCAAAAAAAAAAAAAAAAAA2300.3783330317635254No Hit
GTATCAACGCAGAGTGAATGGGCATTGCTGAAG1970.3240504663365848No Hit
GCTGAAGACGTACAAAAAAAAAAAAAAAAAAAA1960.322405540111526No Hit
ACGCAGAGTGAATGGGCATTGCTGAAAAAAAAA1730.2845722369351735No Hit
GCTGAAGACAAAAAAAAAAAAAAAAAAAAAAAA1700.27963745825999703No Hit
ACGCAGAGTGAATGGGCATTGCTGAAGAAAAAA1540.25331863865905613No Hit
GTGAATGGGCATTGCTGAAGACAAAAAAAAAAA1500.24673893375882092No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1390.22864474528317405No Hit
GAGTGAATGGGCATTGCTGAAAAAAAAAAAAAA1350.2220650403829388No Hit
ACGCAGAGTGAATGGGCATTGCTGAAGACGTAC1270.2089056305824684No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1240.20397085190729194No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1210.19903607323211556No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1210.19903607323211556No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1180.1941012945569391No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1120.18423173720658628No Hit
GAGTGAATGGGCATTGCTGAAGAAAAAAAAAAA1110.1825868109815275No Hit
GCATTGCTGAAGACAAAAAAAAAAAAAAAAAAA1090.17929695853140987No Hit
TATCAACGCAGAGTGAATGGGCATTGCTGAAAA1090.17929695853140987No Hit
ACGCAGAGTGAATGGGCATTGCTGAAGACAAAA1030.16942740118105704No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA1000.16449262250588062No Hit
GTCTTCAGCAAAAAAAAAAAAAAAAAAAAAAAA940.1546230651555278No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA940.1546230651555278No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA900.14804336025529255No Hit
AACGCAGAGTGAATGGGCATTGCTGAAAAAAAA850.13981872912999851No Hit
GTGAATGGGGCATTGCTGAAAAAAAAAAAAAAA830.1365288766798809No Hit
TATCAACGCAGAGTGAATGGGGCATTGCTGAAG830.1365288766798809No Hit
GTGAATGGGCATTGCTGAAGACGTACAAAAAAA830.1365288766798809No Hit
CCCCCCGCATTGCTGAAGACGTACTCTGCGTTG820.1348839504548221No Hit
GTGAATGGGCATTGCTGAAGACGTACTCAAAAA790.12994917177964568No Hit
GTGAATGGGGCATTGCTGAAGAAAAAAAAAAAA770.12665931932952806No Hit
CCCCCGCATTGCTGAAGACGTACTCTGCGTTGA750.12336946687941046No Hit
GCATTGCTGAAGAAAAAAAAAAAAAAAAAAAAA750.12336946687941046No Hit
GGTATCAACGCAGAGTGAATGGGCATTGCTGAA740.12172454065435166No Hit
GAGTACGTCTTCAGCAAAAAAAAAAAAAAAAAA710.11678976197917525No Hit
AACGCAGAGTGAATGGGCATTGCTGAAGACGTA700.11514483575411642No Hit
AACGCAGAGTGAATGGGCATTGCTGAAGAAAAA680.1118549833039988No Hit
ATCAACGCAGAGTGAATGGGCATTGCTGAAGAC660.10856513085388121No Hit
GTGAATGGGGCATTGCTGAAGACAAAAAAAAAA640.10527527840376359No Hit
GTATCAACGCAGAGTGAATGGGCATTGCTGAAA630.1036303521787048No Hit
GTATCAACGCAGAGTGAATGGGGCATTGCTGAA620.10198542595364597No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACTCT200.00854740127.026
GTATCAT257.7422144E-427.01
AAGACTT200.00854740127.021
ACTTACT200.00854740127.024
CTTACTC200.00854740127.025
TATCATC257.7422144E-427.02
TCGCAGA257.7422144E-427.07
AGACTTA200.00854740127.022
ATCGCAG257.7422144E-427.06
TCATCGC257.7422144E-427.04
GACTTAC200.00854740127.023
ATCATCG300.002240175822.53
GCGTTGA3050.016.81967227
ACGGCAT604.0865655E-415.756
TATCAAC3900.015.5769231
TCTGCGT17600.014.95738727
GTGCATT1201.477747E-814.6257
ATCAACG4250.013.6588242
TTGCATT802.3293152E-413.57
ATGCATT1202.3614302E-713.57
Produced by FastQC (version 0.11.2)