FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate1_69.3520000005b9b8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate1_69.3520000005b9b8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17701
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA960.5423422405513812No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA790.44630246878707414No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA750.4237048754307666No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA630.355912095361844No Hit
GTTCAAGGTAAAAAAAAAAAAAAAAAAAAAAAA610.3446132986836902No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA560.31636630698830576No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT540.30506751031015195No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA480.2711711202756906No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA480.2711711202756906No Hit
CCCCAGTTCAAGGTACGTACTCTGCGTTGATAC450.25422292525846No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA400.22597593356307555No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA370.2090277385458449No Hit
GTTCAAGGAAAAAAAAAAAAAAAAAAAAAAAAA350.1977289418676911No Hit
ACGCAGAGTGAATGGGCCAGTTCAAGGAAAAAA270.15253375515507597No Hit
GTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.1468843568159991No Hit
GTTCAAGGTACGTACTCAAAAAAAAAAAAAAAA260.1468843568159991No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA240.1355855601378453No Hit
GGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAA240.1355855601378453No Hit
GCGTTGATACAAAAAAAAAAAAAAAAAAAAAAA230.12993616179876843No Hit
GTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.12993616179876843No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.12993616179876843No Hit
GGCCAGTTCAAGGTAAAAAAAAAAAAAAAAAAA230.12993616179876843No Hit
ACGCAGAGTGAATGGGCCAGTTCAAGGTAAAAA220.12428676345969156No Hit
GTGAATGGGCCAGTTCAAGGAAAAAAAAAAAAA220.12428676345969156No Hit
TATCAACGCAGAGTGAATGGGCCAGTTCAAGGA210.11863736512061467No Hit
GTGAATGGGCCAGTTCAAGGTAAAAAAAAAAAA210.11863736512061467No Hit
ACGCAGAGTGAATGGGCCAGTTCAAAAAAAAAA210.11863736512061467No Hit
AACGCAGAGTGAATGGGCCAGTTCAAGGTAAAA210.11863736512061467No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT200.11298796678153777No Hit
ACTCTGCGTTGATACAAAAAAAAAAAAAAAAAA200.11298796678153777No Hit
GAATGGGCCAGTTCAAGGTAAAAAAAAAAAAAA190.10733856844246088No Hit
GGTACGTACTCTGCGTTGATACAAAAAAAAAAA180.10168917010338399No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCGT5600.019.28571527
CCACCAG350.005324872719.2857156
CACCAGT951.1646989E-817.0526317
ATGGGGA500.002132311716.25
CCCCAGT1157.1540853E-915.260877
CTCTGCG7050.015.12765926
TATCAAC902.245315E-615.0000011
ACCAGTT2350.014.936178
AGCAGTG550.00402592214.7272732
GCAGTGG550.00402592214.7272733
AAGCAGT550.00402592214.7272731
TACTCTG7500.014.2224
TCAACGC953.9384104E-614.2105263
GTACTCT7550.014.12582923
ACTCTGC7550.014.12582925
ACGTACT7900.013.84177221
GGTACGT8200.013.829267518
CGTACTC7850.013.75796122
AAGGTAC8100.013.66666716
AGGTACG8100.013.66666717
Produced by FastQC (version 0.11.2)