FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate1_68.3520000005b99f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate1_68.3520000005b99f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19922
Sequences flagged as poor quality0
Sequence length33
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTAGGAGACTACGTACTCTGCGTTGATACCA2891.450657564501556No Hit
GTAGGAGACTACGTACTCTGCGTTGATACCACT2301.1545025599839374No Hit
GTGAATGGGACGTAGGAGACAAAAAAAAAAAAA1340.6726232305993374No Hit
ACGTAGGAGACAAAAAAAAAAAAAAAAAAAAAA1330.6676036542515812No Hit
ACGTAGGAGAAAAAAAAAAAAAAAAAAAAAAAA1320.6625840779038249No Hit
GTGAATGGGACGTAGGAGAAAAAAAAAAAAAAA1040.5220359401666499No Hit
ACGCAGAGTGAATGGGACGTAGGAGAAAAAAAA1020.5119967874711374No Hit
ACGCAGAGTGAATGGGACGTAGGAGACAAAAAA940.4718401766890874No Hit
GGACGTAGGAGACAAAAAAAAAAAAAAAAAAAA930.46682060034133116No Hit
GGAGACAAAAAAAAAAAAAAAAAAAAAAAAAAA840.4216444132115249No Hit
GGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA700.35137034434293746No Hit
GAGTGAATGGGACGTAGGAGACAAAAAAAAAAA600.30117458086537496No Hit
GGACGTAGGAGAAAAAAAAAAAAAAAAAAAAAA530.2660375464310812No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA520.26101797008332495No Hit
GAGTGAATGGGACGTAGGAGAAAAAAAAAAAAA510.2559983937355687No Hit
GACGTAGGAGACAAAAAAAAAAAAAAAAAAAAA470.2359200883445437No Hit
CGTAGGAGACTACGTACTCTGCGTTGATACCAC470.2359200883445437No Hit
TATCAACGCAGAGTGAATGGGACGTAGGAGACA470.2359200883445437No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA460.2309005119967875No Hit
GTAGGAGACAAAAAAAAAAAAAAAAAAAAAAAA450.22588093564903122No Hit
TATCAACGCAGAGTGAATGGGACGTAGGAGAAA450.22588093564903122No Hit
GAACGTAGGAGACAAAAAAAAAAAAAAAAAAAA450.22588093564903122No Hit
AACGCAGAGTGAATGGGACGTAGGAGACAAAAA430.21584178295351872No Hit
AACGCAGAGTGAATGGGACGTAGGAGAAAAAAA390.19576347756249374No Hit
GTGAATGGGGACGTAGGAGAAAAAAAAAAAAAA380.19074390121473747No Hit
GAATGGGACGTAGGAGACAAAAAAAAAAAAAAA350.17568517217146873No Hit
GTGAATGGGACGTAGGAAAAAAAAAAAAAAAAA330.16564601947595622No Hit
GAATGGGACGTAGGAGAAAAAAAAAAAAAAAAA320.16062644312819999No Hit
GTGAATGGGAACGTAGGAGACAAAAAAAAAAAA310.15560686678044372No Hit
GAGACAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.14556771408493124No Hit
GACGTAGGAGAAAAAAAAAAAAAAAAAAAAAAA270.13552856138941874No Hit
GTATCAACGCAGAGTGAATGGGACGTAGGAGAC260.13050898504166247No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA260.13050898504166247No Hit
GTGAATGGGGACGTAGGAGACAAAAAAAAAAAA260.13050898504166247No Hit
GAACGTAGGAGAAAAAAAAAAAAAAAAAAAAAA260.13050898504166247No Hit
ACGCAGAGTGAATGGGACGTAGGAAAAAAAAAA250.12548940869390623No Hit
GTGAATGGGAACGTAGGAGAAAAAAAAAAAAAA240.12046983234615No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.11545025599839374No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA230.11545025599839374No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA230.11545025599839374No Hit
CAACGCAGAGTGAATGGGACGTAGGAGACAAAA220.11043067965063749No Hit
GGACAACGTAGGAGAAAAAAAAAAAAAAAAAAA220.11043067965063749No Hit
AACGCAGAGTGAATGGGACGTAGGAAAAAAAAA220.11043067965063749No Hit
TATCAACGCAGAGTGAATGGGACGTAGGAAAAA210.10541110330288123No Hit
CAACGCAGAGTGAATGGGACGTAGGAGAAAAAA210.10541110330288123No Hit
GTGAATGGGGACGTAGGAAAAAAAAAAAAAAAA210.10541110330288123No Hit
GAACAACGTAGGAGAAAAAAAAAAAAAAAAAAA200.10039152695512497No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA200.10039152695512497No Hit
CACGTAGGAGACTACGTACTCTGCGTTGATACC200.10039152695512497No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACTCA300.002187933322.5000021
GATACCA706.872142E-921.21428727
AACTCAG350.005347948519.2857152
ACTCAGA350.005347948519.2857153
TCAGAGT350.005347948519.2857155
CTCAGAG350.005347948519.2857154
TATCAAC1300.018.6923071
TGATACC753.387686E-718.026
ATCAACG1600.016.031252
TCAACGC1600.015.18753
GTTGATA953.9993683E-614.21052624
TTGATAC953.9993683E-614.21052625
CGTTGAT956.437429E-512.78947423
CAACGCA1952.5465852E-1112.4615384
AACGCAG2304.638423E-1010.5652175
TGCGTTG2101.4601028E-810.28571427
ACGCAGA3700.09.4864861
GCGTTGA1300.00117918469.34615322
CGCAGAG3551.8189894E-129.12676052
CTGCGTT2401.15636794E-79.00000126
Produced by FastQC (version 0.11.2)