FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate1_66.3520000005b95c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate1_66.3520000005b95c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15423
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAATGGGGTATCCAAAAAAAAAAAAAAAAAA3011.9516306814497828No Hit
GTGAATGGGTATCCAAAAAAAAAAAAAAAAAAA2461.5950204240420152No Hit
GTGAATGGGTATCCAATGCACAAAAAAAAAAAA1460.9466381378460741No Hit
GTATCCAATGCACGTACTCTGCGTTGATACCAC960.6224469947481035No Hit
GTGAATGGGTATCCAATGCAAAAAAAAAAAAAA790.5122220060947935No Hit
GTGAATGGGTATCCAATGAAAAAAAAAAAAAAA760.49277053750891525No Hit
GTGAATGGGTATCCAATGCACGTACTCAAAAAA730.47331906892303705No Hit
TATCAACGCAGAGTGAATGGGTATCCAATGCAC670.4344161317512805No Hit
ACGCAGAGTGAATGGGGTATCCAAAAAAAAAAA670.4344161317512805No Hit
GTGAATGGGTATCCAATGCACGTAAAAAAAAAA650.42144848602736174No Hit
GTGAATGGGTATCCAATGCACGTACAAAAAAAA640.4149646631654023No Hit
GGTATCCAATGCACGTACTCTGCGTTGATACCA630.40848084030344295No Hit
CCGGTATCCAATGCACGTACTCTGCGTTGATAC620.40199701744148353No Hit
CCAATGCACGTACTCAAAAAAAAAAAAAAAAAA570.3695779031316865No Hit
GAGTGAATGGGGTATCCAAAAAAAAAAAAAAAA530.3436426116838488No Hit
AACGCAGAGTGAATGGGGTATCCAAAAAAAAAA450.2917720287881735No Hit
TATCAACGCAGAGTGAATGGGGTATCCAAAAAA450.2917720287881735No Hit
GTGAATGGGGTATCCAATGCACGTACTCAAAAA430.2788043830642547No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA430.2788043830642547No Hit
ACGCAGAGTGAATGGGTATCCAAAAAAAAAAAA430.2788043830642547No Hit
GTGAATGGGGGTATCCAAAAAAAAAAAAAAAAA420.2723205602022953No Hit
ACGCAGAGTGAATGGGTATCCAATGCACAAAAA380.24638526875445763No Hit
GTATCAACGCAGAGTGAATGGGTATCCAATGCA380.24638526875445763No Hit
GGGGTATCCAATGCACGTACTCTGCGTTGATAC340.22044997730662No Hit
CCAATGCACAAAAAAAAAAAAAAAAAAAAAAAA340.22044997730662No Hit
GTGAATGGGGTATCCAATGCACAAAAAAAAAAA330.21396615444466055No Hit
GTGAATGGGTATCCAATGCACGAAAAAAAAAAA330.21396615444466055No Hit
GAATGGGGTATCCAAAAAAAAAAAAAAAAAAAA310.20099850872074176No Hit
GGGTATCCAATGCACGTACTCTGCGTTGATACC300.19451468585878234No Hit
CGGGTATCCAATGCACGTACTCTGCGTTGATAC290.18803086299682292No Hit
GTGAATGGGTATCCAATAAAAAAAAAAAAAAAA280.1815470401348635No Hit
AACGCAGAGTGAATGGGTATCCAAAAAAAAAAA270.17506321727290408No Hit
GCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA270.17506321727290408No Hit
GTGAATGGGGTATCCAATGCACGTAAAAAAAAA260.1685793944109447No Hit
GTATCCAAAAAAAAAAAAAAAAAAAAAAAAAAA250.16209557154898527No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA240.15561174868702587No Hit
CGGTATCCAATGCACGTACTCTGCGTTGATACC240.15561174868702587No Hit
TATCCAATGCACGTACTCTGCGTTGATACCACT240.15561174868702587No Hit
CACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA240.15561174868702587No Hit
CCAATGCACGTACAAAAAAAAAAAAAAAAAAAA240.15561174868702587No Hit
ACGCAGAGTGAATGGGTATCCAATGCACGTACT230.14912792582506645No Hit
GAGTGAATGGGTATCCAAAAAAAAAAAAAAAAA230.14912792582506645No Hit
ATCCAATGCACGTACAAAAAAAAAAAAAAAAAA230.14912792582506645No Hit
ATCCAATGCACAAAAAAAAAAAAAAAAAAAAAA230.14912792582506645No Hit
TATCAACGCAGAGTGAATGGGGTATCCAATGCA230.14912792582506645No Hit
ACGGTATCCAATGCACGTACTCTGCGTTGATAC230.14912792582506645No Hit
TATCAACGCAGAGTGAATGGGTATCCAAAAAAA230.14912792582506645No Hit
GTGAATGGGGTATCCAATGCACGTACAAAAAAA220.14264410296310703No Hit
AACGCAGAGTGAATGGGGTATCCAATGCACGTA220.14264410296310703No Hit
CTGGTATCCAATGCACGTACTCTGCGTTGATAC220.14264410296310703No Hit
GTATCAACGCAGAGTGAATGGGGTATCCAAAAA210.13616028010114764No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA210.13616028010114764No Hit
ACGCAGAGTGAATGGGGTATCCAATGCACGTAC200.12967645723918822No Hit
GTGAATGGGGTATCCAATGCACGTACTCTGCGT200.12967645723918822No Hit
ATGCACGTACTCAAAAAAAAAAAAAAAAAAAAA190.12319263437722881No Hit
ACGCAGAGTGAATGGGTATCCAATGCAAAAAAA190.12319263437722881No Hit
GTGAATGGGGTATCCAATAAAAAAAAAAAAAAA190.12319263437722881No Hit
GAATGGGGGTATCCAAAAAAAAAAAAAAAAAAA190.12319263437722881No Hit
TCGGTATCCAATGCACGTACTCTGCGTTGATAC190.12319263437722881No Hit
GTGAATGGGGAATCCAAAAAAAAAAAAAAAAAA190.12319263437722881No Hit
GTGAATGGGGTATCCAATGAAAAAAAAAAAAAA180.11670881151526939No Hit
ATCAACGCAGAGTGAATGGGTATCCAAAAAAAA170.11022498865331No Hit
GGGTATCCAAAAAAAAAAAAAAAAAAAAAAAAA170.11022498865331No Hit
ATCCAATGCACGTAAAAAAAAAAAAAAAAAAAA170.11022498865331No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.11022498865331No Hit
GAGTGAATGGGTATCCAATGCACGTACAAAAAA170.11022498865331No Hit
CAACGCAGAGTGAATGGGGTATCCAAAAAAAAA160.10374116579135058No Hit
GTATCAACGCAGAGTGAATGGGGTATCCAATGC160.10374116579135058No Hit
GTGAATGGGGTATCAAAAAAAAAAAAAAAAAAA160.10374116579135058No Hit
ACGCAGAGTGAATGGGTATCCAATGAAAAAAAA160.10374116579135058No Hit
GCGGTATCCAATGCACGTACTCTGCGTTGATAC160.10374116579135058No Hit
ACGCAGAGTGAATGGGGGTATCCAAAAAAAAAA160.10374116579135058No Hit
AACGCAGAGTGAATGGGTATCCAATGCACGTAC160.10374116579135058No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG200.00839243226.973942
CTGGTAT350.00532267519.2671017
TATCAAC601.857052E-517.9826282
TCTGCGT3550.017.53318627
TCAACGC653.695368E-516.5993484
ATCAACG653.695368E-516.5993483
CGGGTAT603.8716564E-415.7347997
TGGGGTA1402.1827873E-1115.4136816
ATGGGGT1503.6379788E-1215.28523355
ATGGGTA1950.013.832795
CTCTGCG4500.013.53104626
AATGGGT2050.013.158024
TGGGTAT1951.8189894E-1213.141156
CAACGCA853.6319517E-412.6936195
ACTCTGC4850.012.55457825
TACTCTG5000.012.17794224
AATGGGG2450.012.1107494
GGGGTAT1902.0918378E-1012.067297
GTGAATG5050.012.0180921
TGAATGG5050.012.0180922
Produced by FastQC (version 0.11.2)