FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate1_65.3520000005b932.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate1_65.3520000005b932.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2100
Sequences flagged as poor quality0
Sequence length33
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATACGCACGTTACGTACTCTGCGTTGATACCA160.7619047619047619No Hit
ATACGCACGTTACGTACTCTGCGTTGATACCAC150.7142857142857143No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA140.6666666666666667No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA110.5238095238095238No Hit
CCATACGCACGTTACGTACTCTGCGTTGATACC100.4761904761904762No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA100.4761904761904762No Hit
CATACGCACAAAAAAAAAAAAAAAAAAAAAAAA90.4285714285714286No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.38095238095238093No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT70.33333333333333337No Hit
GGACATACGCAAAAAAAAAAAAAAAAAAAAAAA70.33333333333333337No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA70.33333333333333337No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA70.33333333333333337No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA70.33333333333333337No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA60.2857142857142857No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA60.2857142857142857No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA60.2857142857142857No Hit
GTGAATGGGGCATACGCAAAAAAAAAAAAAAAA60.2857142857142857No Hit
GTGAATGGGCATACGCAAAAAAAAAAAAAAAAA60.2857142857142857No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA50.2380952380952381No Hit
CCCATACGCACGTTACGTACTCTGCGTTGATAC50.2380952380952381No Hit
CGTTACGTACTCAAAAAAAAAAAAAAAAAAAAA40.19047619047619047No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.19047619047619047No Hit
GTGAATGGGCATACGCACAAAAAAAAAAAAAAA30.14285714285714285No Hit
GTGAATGGGCATACGCACGAAAAAAAAAAAAAA30.14285714285714285No Hit
CAACGCAGAGTGAATGGGCCATACGCAAAAAAA30.14285714285714285No Hit
CTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.14285714285714285No Hit
TTTCAATGTAGGGCCAAAAAAAAAAAAAAAAAA30.14285714285714285No Hit
GAGTGAATGGGCATACGCAAAAAAAAAAAAAAA30.14285714285714285No Hit
CTCATACGCACGTTACGTACTCTGCGTTGATAC30.14285714285714285No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA30.14285714285714285No Hit
GTGAATGGGACATACGCAAAAAAAAAAAAAAAA30.14285714285714285No Hit
GCCATACGCAAAAAAAAAAAAAAAAAAAAAAAA30.14285714285714285No Hit
AATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA30.14285714285714285No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTAAAAAAA30.14285714285714285No Hit
GTATCAACGCAGAGTGAATGGGCCATACGCACG30.14285714285714285No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)