FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate1_61.3520000005b8ad.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate1_61.3520000005b8ad.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14401
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATAGAGTCAAAAAAAAAAAAAAAAAAAAAAA1130.7846677314075411No Hit
CCATAGAGAAAAAAAAAAAAAAAAAAAAAAAAA980.6805082980348587No Hit
CCACCATAGAGTCACGTACTCTGCGTTGATACC850.5902367891118673No Hit
ACCATAGAGTCACGTACTCTGCGTTGATACCAC840.5832928268870217No Hit
GCACCATAGAGTCAAAAAAAAAAAAAAAAAAAA650.45135754461495725No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA510.35414207346712034No Hit
ACACCATAGAGTCACGTACTCTGCGTTGATACC490.34025414901742934No Hit
CCATAGAGTAAAAAAAAAAAAAAAAAAAAAAAA460.3194222623428929No Hit
GTGAATGGGCACCATAGAGTCAAAAAAAAAAAA450.31247830011804734No Hit
GCACCATAGAGTCACGTACTCTGCGTTGATACC450.31247830011804734No Hit
GAGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAA450.31247830011804734No Hit
CCATAGAAAAAAAAAAAAAAAAAAAAAAAAAAA430.2985903756683564No Hit
CCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAA410.2847024512186654No Hit
CCATAGAGTCACGTACTCAAAAAAAAAAAAAAA410.2847024512186654No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA400.2777584889938199No Hit
ACGCAGAGTGAATGGGCACCATAGAGAAAAAAA390.2708145267689744No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA380.2638705645441289No Hit
GTGAATGGGCACCATAGAAAAAAAAAAAAAAAA370.2569266023192834No Hit
GAGTGAATGGGCACCATAGAGAAAAAAAAAAAA370.2569266023192834No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA370.2569266023192834No Hit
GTGAATGGGCACCATAGAGAAAAAAAAAAAAAA360.2499826400944379No Hit
ACGCAGAGTGAATGGGCACCATAGAAAAAAAAA360.2499826400944379No Hit
GCACCATAGAGAAAAAAAAAAAAAAAAAAAAAA340.2360947156447469No Hit
GAGTGAATGGGCACCATAGAAAAAAAAAAAAAA330.2291507534199014No Hit
TATCAACGCAGAGTGAATGGGCACCATAGAGTC320.22220679119505587No Hit
GAGTGAATGGGCACCATAGAGTCAAAAAAAAAA300.2083188667453649No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT300.2083188667453649No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA290.20137490452051943No Hit
GCACCATAGAGTAAAAAAAAAAAAAAAAAAAAA280.1944309422956739No Hit
TATCAACGCAGAGTGAATGGGCACCATAGAGAA280.1944309422956739No Hit
GAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.1944309422956739No Hit
ACGCAGAGTGAATGGGCACCATAGAGTCAAAAA250.17359905562113742No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA240.16665509339629192No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA240.16665509339629192No Hit
AACGCAGAGTGAATGGGCACCATAGAAAAAAAA230.15971113117144645No Hit
GTGAATGGGCACCATAGAGTAAAAAAAAAAAAA230.15971113117144645No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA220.15276716894660092No Hit
TCACCATAGAGTCACGTACTCTGCGTTGATACC220.15276716894660092No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.15276716894660092No Hit
CACCATAGAGTCACGTACTCTGCGTTGATACCA210.14582320672175542No Hit
CCACCATAGAGAAAAAAAAAAAAAAAAAAAAAA210.14582320672175542No Hit
GAGTGAATGGGCACCATAGAGTAAAAAAAAAAA200.13887924449690994No Hit
GTCACGTACTCAAAAAAAAAAAAAAAAAAAAAA190.13193528227206444No Hit
AACGCAGAGTGAATGGGCACCATAGAGAAAAAA190.13193528227206444No Hit
GTATCAACGCAGAGTGAATGGGACACCATAGAG180.12499132004721895No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA180.12499132004721895No Hit
GTGAATGGGGCACCATAGAGAAAAAAAAAAAAA180.12499132004721895No Hit
GTATCAACGCAGAGTGAATGGGCACCATAGAGT170.11804735782237345No Hit
TATCAACGCAGAGTGAATGGGCACCATAGAAAA170.11804735782237345No Hit
GTGAATGGGACACCATAGAGAAAAAAAAAAAAA170.11804735782237345No Hit
TATCAACGCAGAGTGAATGGGCACCATAGAGTA170.11804735782237345No Hit
GAGTACGTGACTCTAAAAAAAAAAAAAAAAAAA160.11110339559752794No Hit
GGTATCAACGCAGAGTGAATGGGCACCATAGAG160.11110339559752794No Hit
GTGAATGGGACACCATAGAAAAAAAAAAAAAAA160.11110339559752794No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.10415943337268245No Hit
ATCAACGCAGAGTGAATGGGCACCATAGAGTCA150.10415943337268245No Hit
GTGAATGGGGAGTACGTGACTCTAAAAAAAAAA150.10415943337268245No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.10415943337268245No Hit
ACGCAGAGTGAATGGGCACCATAGAGTAAAAAA150.10415943337268245No Hit
GTGAATGGGGCACCAAAAAAAAAAAAAAAAAAA150.10415943337268245No Hit
ACACCATAGAGAAAAAAAAAAAAAAAAAAAAAA150.10415943337268245No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTTG1401.8189894E-1216.39285727
ACTCTAT905.790826E-412.01
TATCAAC1254.744101E-611.8799992
CTATGGT800.00335904111.81254
CTGCGTT2057.0940587E-1011.19512226
TATGGTG850.005284677311.1176475
TCTATGG900.008086923510.53
CTCTATG1050.0021056410.2857142
TCAACGC1503.592333E-59.93
ATCAACG1503.592333E-59.93
GTATCAA1555.153186E-59.5806451
TCTGCGT3055.2750693E-119.29508227
CAACGCA1802.633041E-48.254
CTCTGCG3702.419256E-97.66216226
ACTCTGC3753.1450327E-97.559999525
TACTCTG3804.0727173E-97.460526524
GTACTCT3906.7539077E-97.26923123
ACACCAT1950.00541785556.9230778
ACGTACT4101.7796992E-86.914633821
CGTACTC4101.7796992E-86.914633822
Produced by FastQC (version 0.11.2)