FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate1_60.3520000005b883.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate1_60.3520000005b883.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18725
Sequences flagged as poor quality0
Sequence length33
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTAAGGATTCACGTACTCTGCGTTGATACCA2011.0734312416555407No Hit
GTGAATGGGCGTAAGGATAAAAAAAAAAAAAAA1911.020026702269693No Hit
GGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1750.9345794392523363No Hit
GTGAATGGGCGTAAGGAAAAAAAAAAAAAAAAA1560.8331108144192255No Hit
ACGCAGAGTGAATGGGCGTAAGGAAAAAAAAAA1000.5340453938584779No Hit
CACGTACTCTGCGAAAAAAAAAAAAAAAAAAAA920.49132176234979974No Hit
ACGCAGAGTGAATGGGCGTAAGGATAAAAAAAA890.4753004005340454No Hit
GAGTGAATGGGCGTAAGGATAAAAAAAAAAAAA890.4753004005340454No Hit
GCGTAAGGATAAAAAAAAAAAAAAAAAAAAAAA890.4753004005340454No Hit
GTGAATGGGGCGTAAGGATAAAAAAAAAAAAAA770.411214953271028No Hit
CACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA750.4005340453938585No Hit
GGATTCACGTACTCAAAAAAAAAAAAAAAAAAA700.3738317757009346No Hit
GTGAATGGGGCGTAAGGAAAAAAAAAAAAAAAA700.3738317757009346No Hit
AACGCAGAGTGAATGGGCGTAAGGATAAAAAAA680.36315086782376504No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA670.35781041388518026No Hit
TATCAACGCAGAGTGAATGGGATTCACGTACTC650.3471295060080107No Hit
TATCAACGCAGAGTGAATGGGCGTAAGGATAAA640.34178905206942595No Hit
TATCAACGCAGAGTGAATGGGCGTAAGGAAAAA630.3364485981308411No Hit
GGCGTAAGGATAAAAAAAAAAAAAAAAAAAAAA610.3257676902536716No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA560.29906542056074764No Hit
GAGTGAATGGGCGTAAGGAAAAAAAAAAAAAAA530.28304405874499333No Hit
AACGCAGAGTGAATGGGCGTAAGGAAAAAAAAA510.27236315086782376No Hit
GAGTGAATGGGGCGTAAGGATAAAAAAAAAAAA460.24566088117489987No Hit
GTATCAACGCAGAGTGAATGGGCGTAAGGATAA450.2403204272363151No Hit
GTATCAACGCAGAGTGAATGGGATTCACGTACT380.20293724966622162No Hit
ACGCAGAGTGAATGGGGCGTAAGGATAAAAAAA370.19759679572763683No Hit
ACGCAGAGTGAATGGGGCGTAAGGAAAAAAAAA370.19759679572763683No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA360.19225634178905207No Hit
ATTCACGTACTCTGCGAAAAAAAAAAAAAAAAA360.19225634178905207No Hit
GGCGTAAGGAAAAAAAAAAAAAAAAAAAAAAAA340.18157543391188252No Hit
GATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA330.17623497997329773No Hit
CGTAAGGATTCACGTACTCTGCGTTGATACCAC330.17623497997329773No Hit
GAATGGGCGTAAGGAAAAAAAAAAAAAAAAAAA330.17623497997329773No Hit
GAATGGGCGTAAGGATAAAAAAAAAAAAAAAAA330.17623497997329773No Hit
GTTTAAGCATATCAAAAAAAAAAAAAAAAAAAA320.17089452603471297No Hit
GCGTAAGGAAAAAAAAAAAAAAAAAAAAAAAAA320.17089452603471297No Hit
GAGTGAATGGGGCGTAAGGAAAAAAAAAAAAAA320.17089452603471297No Hit
CGTAAGGATAAAAAAAAAAAAAAAAAAAAAAAA320.17089452603471297No Hit
GATTCACGTACTCAAAAAAAAAAAAAAAAAAAA310.16555407209612816No Hit
CGCGTAAGGATTCACGTACTCTGCGTTGATACC310.16555407209612816No Hit
GGCGTAAGGATTCACGTACTCTGCGTTGATACC310.16555407209612816No Hit
ACGCAGAGTGAATGGGATTCACGTACTCTGCGA290.1548731642189586No Hit
ATCAACGCAGAGTGAATGGGCGTAAGGATAAAA290.1548731642189586No Hit
GTGAATGGGATTCACGTACTCTGCGAAAAAAAA280.14953271028037382No Hit
GTATCAACGCAGAGTGAATGGGCGTAAGGAAAA250.13351134846461948No Hit
CTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.13351134846461948No Hit
ATCAACGCAGAGTGAATGGGCGTAAGGAAAAAA250.13351134846461948No Hit
GTGAATGGGGGCGTAAGGATAAAAAAAAAAAAA240.12817089452603472No Hit
TTCACGTACTCTGCGAAAAAAAAAAAAAAAAAA240.12817089452603472No Hit
GTGAATGGGCGTAAAAAAAAAAAAAAAAAAAAA240.12817089452603472No Hit
GAGTGAATGGGATTCACGTACTCTGCGAAAAAA240.12817089452603472No Hit
GAATGGGGCGTAAGGATAAAAAAAAAAAAAAAA240.12817089452603472No Hit
CAACGCAGAGTGAATGGGCGTAAGGATAAAAAA240.12817089452603472No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA230.12283044058744993No Hit
ACGCAGAGTGAATGGGCGTAAAAAAAAAAAAAA220.11748998664886515No Hit
GTGAATGGGAGCGTAAGGATAAAAAAAAAAAAA220.11748998664886515No Hit
ACGCAGAGTGAATGGGATTCACGTACTCTGCGT210.11214953271028037No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.11214953271028037No Hit
GAATGGGGCGTAAGGAAAAAAAAAAAAAAAAAA210.11214953271028037No Hit
ACGCAGAGTGAATGGGCGTAAGAAAAAAAAAAA210.11214953271028037No Hit
CACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA200.1068090787716956No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA200.1068090787716956No Hit
TATCAACGCAGAGTGAATGGGGCGTAAGGAAAA200.1068090787716956No Hit
AACGCAGAGTGAATGGGGCGTAAGGAAAAAAAA200.1068090787716956No Hit
AACGCAGAGTGAATGGGATTCACGTACTCTGCG190.10146862483311081No Hit
CAACGCAGAGTGAATGGGCGTAAGGAAAAAAAA190.10146862483311081No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT190.10146862483311081No Hit
TATCAACGCAGAGTGAATGGGGCGTAAGGATAA190.10146862483311081No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA757.025921E-616.1582891
TCTGCGA603.8969115E-415.7515648
TGCGTTG902.2590502E-615.00148927
CTGCGAA657.159538E-414.5399059
TATCAAC1800.014.2133081
TGCGAAA700.001254396713.5013410
TCTGCGT2600.012.46277527
TCAACGC2003.8198777E-1112.1512073
ATCAACG2003.8198777E-1112.1512072
CTGCGTT1152.8419321E-511.74029526
GTATCAA1153.7885565E-410.5662662
CAACGCA2304.5292836E-1010.5662664
CTCTGCG3750.09.00089426
CAGAGTG3900.09.0008944
GCAGAGT3900.09.0008943
ACGCAGA4050.08.9768271
AGAGTGA4100.08.8911285
AACGCAG2759.984433E-98.837245
CGCAGAG4150.08.7840042
AGTGAAT4750.07.95868547
Produced by FastQC (version 0.11.2)