FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate1_6.3520000005b239.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate1_6.3520000005b239.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47710
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTACGATCAGACGTACTCTGCGTTGATACCAC5201.08991825613079No Hit
CCCCAGGTCAGGCGGGATTACCCGCTGAGTTTA2810.5889750576399078No Hit
GTGCTACGATCAGACGTACTCTGCGTTGATACC2450.5135191783693146No Hit
GTTTAAGCATATCAAAAAAAAAAAAAAAAAAAA2370.4967512051980717No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1820.38147138964577654No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1760.3688954097673444No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1750.366799413120939No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1650.3458394466568854No Hit
CGTCTGATCGTAGCAAAAAAAAAAAAAAAAAAA1580.3311674701320478No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1550.3248794801928317No Hit
GAGTTTAAGCATATCAAAAAAAAAAAAAAAAAA1440.3018235170823727No Hit
GATCAGACGTACTCAAAAAAAAAAAAAAAAAAA1400.2934395304967512No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA1250.2619995808006707No Hit
CTGCTACGATCAGACGTACTCTGCGTTGATACC1210.25361559421504926No Hit
GGATTACCCGCTGAGTTTAAGCATATCAAAAAA1160.24313561098302244No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA1060.22217564451896876No Hit
GGTCAGGCGGGATTACCCGCTGAGTTTAAGCAT970.2033116747013205No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA960.2012156780549151No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA950.19911968140850972No Hit
ACCCCAGGTCAGGCGGGATTACCCGCTGAGTTT900.1886396981764829No Hit
GTGAATGGGGCGACCCCAGGTCAGGCGGGATTA900.1886396981764829No Hit
GAGTACGTCTGATCGTAGCAAAAAAAAAAAAAA860.18025571159086146No Hit
GACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA800.16767973171242925No Hit
CCCCTGCTACGATCAGACGTACTCTGCGTTGAT760.1592957451268078No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA740.15510375183399708No Hit
ATGCTACGATCAGACGTACTCTGCGTTGATACC740.15510375183399708No Hit
GACGTACTCTGCGAAAAAAAAAAAAAAAAAAAA710.14881576189478096No Hit
GACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA670.1404317753091595No Hit
GCTACGATCAGACGTACTCTGCGTTTATACCAC660.13833577866275412No Hit
GGTCTGATCGTAGCAAAAAAAAAAAAAAAAAAA620.12995179207713267No Hit
GTGCTACAAAAAAAAAAAAAAAAAAAAAAAAAA610.1278557954307273No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA600.12575979878432195No Hit
GTGAATGGGGTCTGATCGTAGCAAAAAAAAAAA570.11947180884510586No Hit
GGATTACCCGCTGAGTTTAAGCATATCAATAAG550.1152798155522951No Hit
TGCTACGATCAGACGTACTCTGCGTTGATACCA540.11318381890588974No Hit
GTGAATGGGGAGTACGTCTGATCGTAGCAAAAA510.10689582896667366No Hit
GTGAATGGGGGCGTGCAAAAAAAAAAAAAAAAA480.10060783902745755No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTACG200.00852925527.02
TCTACGC200.00852925527.03
GTATAAC200.00852925527.01
CTACGCA200.00852925527.04
TGTGCTC200.00852925527.010
TACGCAG257.7176007E-426.9999985
ATACCAC559.353826E-619.63636427
GCGTGCA350.005457005519.28571311
CCCAGGT952.4800465E-715.6315792
CCAGGTC952.4800465E-715.6315793
AGGTCAG1004.4377703E-714.8499995
CAGGTCA1004.4377703E-714.8499994
CCCCAGG1057.706003E-714.1428571
CGTTGAT2650.013.75471827
CGGGATT1152.1464584E-612.91304313
GGGATTA1152.1464584E-612.91304314
TATCAAC3250.012.8769231
ACTGCTA1203.458781E-612.3757
AGGCGGG1255.458467E-611.8810
CAGGCGG1255.458467E-611.889
Produced by FastQC (version 0.11.2)