FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate1_55.3520000005b7e1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate1_55.3520000005b7e1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21305
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAATGGGCATTGCTGAAGAAAAAAAAAAAAA1690.7932410232339827No Hit
GCATTGCTGAAGACGTACTCTGCGTTGATACCA1470.6899788781976062No Hit
ACGCAGAGTGAATGGGCATTGCTGAAAAAAAAA1420.666510208871157No Hit
TATCAACGCAGAGTGAATGGGCATTGCTGAAGA1190.5585543299694907No Hit
ACGCAGAGTGAATGGGCATTGCTGAAGAAAAAA1150.5397793945083313No Hit
GTGAATGGGCATTGCTGAAAAAAAAAAAAAAAA1140.5350856606430415No Hit
GCTGAAGACGTACTCAAAAAAAAAAAAAAAAAA1120.5256981929124618No Hit
GCTGAAGACGTACAAAAAAAAAAAAAAAAAAAA950.4459047172025346No Hit
GTATCAACGCAGAGTGAATGGGCATTGCTGAAG880.4130485801455057No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA820.38488617695376676No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA780.3661112414926074No Hit
GCTGAAGACAAAAAAAAAAAAAAAAAAAAAAAA720.3379488383008683No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA710.3332551044355785No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA680.31917390283970903No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT660.30978643510912934No Hit
ACGCAGAGTGAATGGGCATTGCTGAAGACGTAC650.30509270124383947No Hit
GTGAATGGGCATTGCTGAAGACAAAAAAAAAAA630.2957052335132598No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA600.28162403191739027No Hit
TATCAACGCAGAGTGAATGGGCATTGCTGAAAA570.26754283032152076No Hit
GAGTGAATGGGCATTGCTGAAAAAAAAAAAAAA560.2628490964562309No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA550.2581553625909411No Hit
ACGCAGAGTGAATGGGCATTGCTGAAGACAAAA510.23938042712978175No Hit
AACGCAGAGTGAATGGGCATTGCTGAAAAAAAA510.23938042712978175No Hit
GCATTGCTGAAGACAAAAAAAAAAAAAAAAAAA500.23468669326449193No Hit
GAGTGAATGGGCATTGCTGAAGAAAAAAAAAAA500.23468669326449193No Hit
ATCAACGCAGAGTGAATGGGCATTGCTGAAGAC480.22529922553391224No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA470.22060549166862237No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA460.21591175780333255No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA460.21591175780333255No Hit
GCATTGCTGAAGAAAAAAAAAAAAAAAAAAAAA450.21121802393804273No Hit
AACGCAGAGTGAATGGGCATTGCTGAAGAAAAA420.1971368223421732No Hit
AACGCAGAGTGAATGGGCATTGCTGAAGACGTA370.173668153015724No Hit
GTGAATGGGCATTGCTGAAGACGTACTCAAAAA360.16897441915043415No Hit
GGGCATTGCTGAAGAAAAAAAAAAAAAAAAAAA360.16897441915043415No Hit
GTGAATGGGCATTGCTGAAGACGTACAAAAAAA350.16428068528514433No Hit
GTGAATGGGGCATTGCTGAAGAAAAAAAAAAAA340.15958695141985452No Hit
GAGTACGTCTTCAGCAAAAAAAAAAAAAAAAAA330.15489321755456467No Hit
TTGCTGAAGAAAAAAAAAAAAAAAAAAAAAAAA310.14550574982398498No Hit
GCATTGCTGAAAAAAAAAAAAAAAAAAAAAAAA310.14550574982398498No Hit
ATCAACGCAGAGTGAATGGGCATTGCTGAAGAA300.14081201595869514No Hit
GTGAATGGGGCATTGCTGAAAAAAAAAAAAAAA290.1361182820934053No Hit
GAGTGAATGGGCATTGCTGAAGACAAAAAAAAA290.1361182820934053No Hit
ATCAACGCAGAGTGAATGGGCATTGCTGAAAAA280.13142454822811545No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA270.12673081436282563No Hit
GTCTTCAGCAAAAAAAAAAAAAAAAAAAAAAAA260.1220370804975358No Hit
GGTATCAACGCAGAGTGAATGGGCATTGCTGAA260.1220370804975358No Hit
GCATTGCTGAAGACGTACTCAAAAAAAAAAAAA260.1220370804975358No Hit
GCATTGCTGAAGACGTAAAAAAAAAAAAAAAAA260.1220370804975358No Hit
GTGAATGGGCATTGCTGAAGACGTAAAAAAAAA250.11734334663224597No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA250.11734334663224597No Hit
GTGAATGGGGCATTGCTGAAGACAAAAAAAAAA250.11734334663224597No Hit
GTATCAACGCAGAGTGAATGGGCATTGCTGAAA240.11264961276695612No Hit
GCATTGCTGAAGACGTACAAAAAAAAAAAAAAA240.11264961276695612No Hit
GAATGGGCATTGCTGAAGAAAAAAAAAAAAAAA230.10795587890166627No Hit
CCGCATTGCTGAAGACGTACTCTGCGTTGATAC230.10795587890166627No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.10795587890166627No Hit
ACGCAGAGTGAATGGGCATTGCTGAAGACGTAA220.10326214503637643No Hit
TATCAACGCAGAGTGAATGGGCATTTCTGAAGA220.10326214503637643No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.10326214503637643No Hit
GCTGAAGAAAAAAAAAAAAAAAAAAAAAAAAAA220.10326214503637643No Hit
GAATGGGAAGCAGTGGTATCAAAAAAAAAAAAA220.10326214503637643No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATT552.0242092E-417.1818187
CAGCATT603.931791E-415.7500017
TCTGCGT5550.015.56756827
GCGCATT707.118729E-515.4285727
TGCGTTG2050.013.829267527
CTCTGCG6400.013.289062526
TATCAAC1551.897206E-913.0645161
ACTCTGC6800.012.70588325
CTGCGTT2300.012.32608726
TACTCTG7050.012.25531924
GCGTTGA1001.0468926E-412.1527
GTATCAA1451.5621845E-712.1034471
GGTATCA800.003453477311.81251
GTACTCT7350.011.75510223
CGCATTG3300.011.4545468
CGTACTC7600.011.36842222
AGCATTG1553.7175596E-711.322588
ACGTACT7750.011.14838721
TGGGGCA1000.00145173910.79999913
GACGTAC8150.010.766870520
Produced by FastQC (version 0.11.2)