FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate1_52.3520000005b791.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate1_52.3520000005b791.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13982
Sequences flagged as poor quality0
Sequence length33
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCGACGTCTACGTACTCTGCGTTGATACCA3702.646259476469747No Hit
GTGAATGGGTATCGACGAAAAAAAAAAAAAAAA1721.230153053926477No Hit
GTGAATGGGTATCGACGTCAAAAAAAAAAAAAA1441.029895580031469No Hit
GTGAATGGGTATCGACGTAAAAAAAAAAAAAAA1350.9655271062795022No Hit
GTATCGACGTCAAAAAAAAAAAAAAAAAAAAAA910.6508367901587756No Hit
ACGCAGAGTGAATGGGTATCGACGAAAAAAAAA730.5220998426548419No Hit
ACGCAGAGTGAATGGGTATCGACGTCAAAAAAA700.5006436847375196No Hit
GTGAATGGGGTATCGACAAAAAAAAAAAAAAAA700.5006436847375196No Hit
GTGAATGGGTATCGACAAAAAAAAAAAAAAAAA690.49349163209841224No Hit
GTATCGACGTAAAAAAAAAAAAAAAAAAAAAAA570.4076670004291232No Hit
GAGTGAATGGGTATCGACGTCAAAAAAAAAAAA560.40051494779001573No Hit
GTGAATGGGTATCGAAAAAAAAAAAAAAAAAAA560.40051494779001573No Hit
ACGCAGAGTGAATGGGTATCGACGTAAAAAAAA550.39336289515090833No Hit
GTGAATGGGGTATCGACGTCAAAAAAAAAAAAA550.39336289515090833No Hit
ACGCAGAGTGAATGGGTATCGACAAAAAAAAAA470.3361464740380489No Hit
GAGTGAATGGGTATCGACGAAAAAAAAAAAAAA460.32899442139894153No Hit
TATCAACGCAGAGTGAATGGGTATCGACGAAAA460.32899442139894153No Hit
TATCAACGCAGAGTGAATGGGTATCGACGTAAA410.29323415820340437No Hit
AACGCAGAGTGAATGGGTATCGACGTCAAAAAA390.2789300529251895No Hit
TATCAACGCAGAGTGAATGGGTATCGACGTCAA390.2789300529251895No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA380.27177800028608207No Hit
AACGCAGAGTGAATGGGTATCGACGAAAAAAAA380.27177800028608207No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA370.26462594764697467No Hit
GAGTGAATGGGTATCGACGTAAAAAAAAAAAAA360.25747389500786727No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA350.2503218423687598No Hit
GTATCGACGAAAAAAAAAAAAAAAAAAAAAAAA330.23601773709054497No Hit
AACGCAGAGTGAATGGGTATCGACGTAAAAAAA330.23601773709054497No Hit
GTGAATGGGGTATCGACGAAAAAAAAAAAAAAA320.22886568445143757No Hit
GTATCGACGTCTACAAAAAAAAAAAAAAAAAAA290.2074095265341153No Hit
GGTATCGACAAAAAAAAAAAAAAAAAAAAAAAA290.2074095265341153No Hit
ACGCAGAGTGAATGGGTATCGAAAAAAAAAAAA280.20025747389500786No Hit
GTACGTAGACGTCGAAAAAAAAAAAAAAAAAAA280.20025747389500786No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.185953368616793No Hit
TATCAACGCAGAGTGAATGGGTATCGACGTCTA260.185953368616793No Hit
GGTATCGACGTCTACGTACTCTGCGTTGATACC250.1788013159776856No Hit
GTGAATGGGTATCGACGTCTACAAAAAAAAAAA250.1788013159776856No Hit
GTATCGACGTCTACGTACAAAAAAAAAAAAAAA250.1788013159776856No Hit
GTATCGACGTCTACGAAAAAAAAAAAAAAAAAA250.1788013159776856No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA240.1716492633385782No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA230.16449721069947076No Hit
GTGAATGGGGTATCGAAAAAAAAAAAAAAAAAA230.16449721069947076No Hit
GTATCGACGTCTACGTAAAAAAAAAAAAAAAAA220.15734515806036334No Hit
ATCAACGCAGAGTGAATGGGTATCGACGTAAAA220.15734515806036334No Hit
GTATCAACGCAGAGTGAATGGGTATCGACGTCA220.15734515806036334No Hit
GTGAATGGGGTATCGACGTAAAAAAAAAAAAAA210.1501931054212559No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA210.1501931054212559No Hit
TCTACGTACTCAAAAAAAAAAAAAAAAAAAAAA210.1501931054212559No Hit
ACGCAGAGTGAATGGGGTATCGACAAAAAAAAA210.1501931054212559No Hit
ACGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.1501931054212559No Hit
GTATCAACGCAGAGTGAATGGGTATCGACGTAA200.14304105278214846No Hit
GTATCAACGCAGAGTGAATGGGTATCGACGAAA190.13588900014304103No Hit
CGTCTACGTACTCAAAAAAAAAAAAAAAAAAAA190.13588900014304103No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT190.13588900014304103No Hit
GTGAATGGGTATCGACGTCTACGTACAAAAAAA190.13588900014304103No Hit
AACGCAGAGTGAATGGGGTATCGACAAAAAAAA190.13588900014304103No Hit
GAGTGAATGGGTATCGAAAAAAAAAAAAAAAAA190.13588900014304103No Hit
AACGCAGAGTGAATGGGTATCGACAAAAAAAAA190.13588900014304103No Hit
ATCAACGCAGAGTGAATGGGTATCGACGAAAAA180.12873694750393364No Hit
GAGTGAATGGGGTATCGACAAAAAAAAAAAAAA180.12873694750393364No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA180.12873694750393364No Hit
TATCAACGCAGAGTGAATGGGGTATCGACGTCT170.1215848948648262No Hit
CGTATCGACGTCTACGTACTCTGCGTTGATACC170.1215848948648262No Hit
CCGTATCGACGTCTACGTACTCTGCGTTGATAC170.1215848948648262No Hit
GTGAATGGGAGTATCGACGAAAAAAAAAAAAAA170.1215848948648262No Hit
TATCAACGCAGAGTGAATGGGTATCGACAAAAA170.1215848948648262No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA160.11443284222571878No Hit
CCCCCGTATCGACGTCTACGTACTCTGCGTTGA160.11443284222571878No Hit
GAGTGAATGGGGTATCGACGTCAAAAAAAAAAA160.11443284222571878No Hit
GTGAATGGGTATCGACGTCTAAAAAAAAAAAAA150.10728078958661136No Hit
ACGCAGAGTGAATGGGGTATCGACGAAAAAAAA150.10728078958661136No Hit
GTGAATGGGGGTATCGAAAAAAAAAAAAAAAAA150.10728078958661136No Hit
CAACGCAGAGTGAATGGGTATCGACGAAAAAAA150.10728078958661136No Hit
GTATCGACGTCTAAAAAAAAAAAAAAAAAAAAA150.10728078958661136No Hit
ACGCAGAGTGAATGGGTATCGACGTCTACGTAC150.10728078958661136No Hit
GAATGGGTATCGACAAAAAAAAAAAAAAAAAAA140.10012873694750393No Hit
TATCAACGCAGAGTGAATGGGTATCGAAAAAAA140.10012873694750393No Hit
ACGCAGAGTGAATGGGGTATCGACGTCAAAAAA140.10012873694750393No Hit
GTGTATCGACGTCTACGTACTCTGCGTTGATAC140.10012873694750393No Hit
GTATCGACGTCTACGTACTCAAAAAAAAAAAAA140.10012873694750393No Hit
GGTATCGACGTCAAAAAAAAAAAAAAAAAAAAA140.10012873694750393No Hit
CAACGCAGAGTGAATGGGTATCGACGTAAAAAA140.10012873694750393No Hit
ATCAACGCAGAGTGAATGGGTATCGACGTCTAC140.10012873694750393No Hit
TATCAACGCAGAGTGAATGGGGTATCGACGAAA140.10012873694750393No Hit
GTGAATGGGTATCGACGTCTACGAAAAAAAAAA140.10012873694750393No Hit
AACGCAGAGTGAATGGGTATCGAAAAAAAAAAA140.10012873694750393No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACCA551.2047167E-824.54545427
TGATACC603.0368028E-822.526
TTGATAC753.206278E-718.00000225
GTTGATA902.1606884E-615.024
TATCAAC953.7909922E-614.2105261
CGTTGAT953.7909922E-614.21052623
TCAACGC1351.3947107E-410.03
ATCAACG1351.3947107E-410.02
GCGTTGA1402.0142198E-49.64285822
ATGGGGT1350.00159867389.05
CAACGCA1651.0165578E-49.03
AACGCAG2451.3959943E-68.2653061
TGCGTTG1750.00186798857.71428621
CTGCGTT1750.00186798857.71428620
AATGGGG2202.67842E-47.36363654
GTGAATG5455.456968E-126.935781
TGAATGG5352.3646862E-116.81308362
ACGCAGA3402.5968984E-66.751
AGTGAAT4051.0888107E-76.66666652
GAGTGAA4151.7017555E-76.50602441
Produced by FastQC (version 0.11.2)