FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate1_51.3520000005b777.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate1_51.3520000005b777.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46330
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCTGTTCCTCACGTACTCTGCGTTGATACCA11582.499460392834017No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA3060.660479171163393No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2650.5719835959421541No Hit
CTCTGTTCCTCACGTACTCTGCGTTGATACCAC2290.4942801640405785No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1600.3453485862292251No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1420.3064968702784373No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA1400.30218001295057195No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA1280.2762788689833801No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA1230.2654867256637168No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1020.22015972372113102No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT970.20936758040146775No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA960.20720915173753507No Hit
CTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA960.20720915173753507No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA850.18346643643427585No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA760.16404057845888195No Hit
CTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA690.14893157781135336No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA630.13598100582775738No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA610.13166414849989208No Hit
GGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAA580.1251888625080941No Hit
GAATGGGAAGCAGTGGTATCAAAAAAAAAAAAA570.12303043384416146No Hit
CTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA560.12087200518022878No Hit
CCACTCTGTTCCTCACGTACTCTGCGTTGATAC510.11007986186056551No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA500.10792143319663286No Hit
GTTCCTCACGTACTCAAAAAAAAAAAAAAAAAA480.10360457586876753No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAGTG200.00852669927.04
TGAGTGA200.00852669927.05
TGATACC1650.019.63636426
GATACCA1750.018.51428627
TTGATAC1850.017.51351425
GTTGATA1950.017.30769224
CTACTCA500.00220404416.24
ATACCAC604.0602885E-415.7527
GCGTTGA5100.015.35294127
CGTTGAT2300.014.67391323
TATCAAC2500.012.961
ATCAACG2950.010.5254242
TGCGTTG7650.010.23529326
TCAACGC3050.010.1803283
CAACGCA3253.092282E-119.1384624
CTGCGTT8650.09.05202325
TACTCAA1350.0017548799.05
TCTGCGT12950.08.96525127
ATGGGGC1556.268137E-48.70967712
TGGGACT1556.268137E-48.7096776
Produced by FastQC (version 0.11.2)