FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate1_50.3520000005b75e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate1_50.3520000005b75e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15123
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1320.8728426899424718No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1320.8728426899424718No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1140.7538186867684984No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA810.5356080142828803No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA790.5223831250413278No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA730.48270845731666995No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT680.4496462342127885No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA600.3967466772465781No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA550.36368445414269657No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA450.29756000793493353No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA390.2578853402102757No Hit
GTAGTGAGCTAAAAAAAAAAAAAAAAAAAAAAA330.21821067248561796No Hit
GAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAA320.21159822786484161No Hit
GAATGGGAAGCAGTGGTATCAAAAAAAAAAAAA310.20498578324406533No Hit
CCCTAGCTCACTACCACGTACTCTGCGTTGATA300.19837333862328904No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA290.19176089400251273No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.1851484493817364No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT230.15208622627785492No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA210.13886133703630232No Hit
GCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.12563644779474972No Hit
GTGAATGGGGTAGCTCAAAAAAAAAAAAAAAAA180.11902400317397342No Hit
GTGAATGGGGTGGTAGTGAGCTAAAAAAAAAAA170.11241155855319712No Hit
CCTTAGCTCACTACCACGTACTCTGCGTTGATA170.11241155855319712No Hit
ATCAACGCAGAGTGAATGGGAAGCAGTGGTATC170.11241155855319712No Hit
GCCTAGCTCACTACCACGTACTCTGCGTTGATA160.10579911393242081No Hit
CAACGCAGAGTGAATGGGAAGCAGTGGTATCAA160.10579911393242081No Hit
GTACGTGGTAGTGAGCTAAAAAAAAAAAAAAAA160.10579911393242081No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATAGC200.00838879226.9735093
GTTGATA551.9399484E-417.22198727
ACTAGCT603.8668292E-415.7345477
CGTTGAT750.002042007412.629456526
TCTGCGT3350.011.30996227
TGCGTTG1751.3156387E-710.8252527
TATCAAC1351.3333486E-410.056791
ATCAACG1351.42758E-49.990192
CTGCGTT1955.847287E-79.71496826
TCAACGC1402.0614233E-49.6333973
CTCTGCG4350.09.02104126
CAGAGTG2401.0822368E-68.4292224
AGAGTGA2401.0822368E-68.4292225
ACTCTGC4500.08.41963925
TACTCTG4500.08.41963924
CAACGCA1450.00312226088.3710894
CGCAGAG2603.6633173E-78.2995412
GTACTCT4600.08.23660323
ACGCAGA2552.345807E-67.98627421
CGTACTC4800.07.893410722
Produced by FastQC (version 0.11.2)