FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate1_5.3520000005b22c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate1_5.3520000005b22c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33548
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTAGCTCACGTACTCTGCGTTGATACCACTG2650.7899129605341599No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1550.4620245618218672No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1390.41433170382735185No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1320.3934660784547514No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1130.33683080958626443No Hit
GTGAATGGGTAGACGTAAAAAAAAAAAAAAAAA1110.33086920233695005No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA1010.3010611660903779No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA980.29211875521640635No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA980.29211875521640635No Hit
GTGAATGGGTAGACGTAGAAAAAAAAAAAAAAA920.2742339334684631No Hit
AGACGTAGCTCACGTACTCTGCGTTGATACCAC910.2712531298438059No Hit
GTTTAAGCATATCAAAAAAAAAAAAAAAAAAAA910.2712531298438059No Hit
CCCCAGGTCAGGCGGGATTACCCGCTGAGTTTA870.2593299153451771No Hit
CCCTAGACGTAGCTCACGTACTCTGCGTTGATA860.25634911172051983No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA850.25336830809586264No Hit
CCCCTAGACGTAGCTCACGTACTCTGCGTTGAT830.24740670084654823No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA820.24442589722189104No Hit
CCTAGACGTAGCTCACGTACTCTGCGTTGATAC800.2384642899725766No Hit
GAGTTTAAGCATATCAAAAAAAAAAAAAAAAAA620.18480982472874688No Hit
GGATTACCCGCTGAGTTTAAGCATATCAAAAAA590.17586741385477525No Hit
GTGAATGGGGTAGACGTAAAAAAAAAAAAAAAA580.17288661023011803No Hit
ACGCAGAGTGAATGGGTAGACGTAAAAAAAAAA570.1699058066054608No Hit
GTGAATGGGGTAGACGAAAAAAAAAAAAAAAAA570.1699058066054608No Hit
GGTAGACGTAGCTCACGTACTCTGCGTTGATAC510.15202098485751758No Hit
GTTAGACGTAGCTCACGTACTCTGCGTTGATAC460.13711696673423154No Hit
GCTAGACGTAGCTCACGTACTCTGCGTTGATAC450.13413616310957435No Hit
ACGCAGAGTGAATGGGTAGACGTAGAAAAAAAA440.13115535948491713No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA430.12817455586025991No Hit
CCCTTAGACGTAGCTCACGTACTCTGCGTTGAT420.12519375223560272No Hit
CCTTAGACGTAGCTCACGTACTCTGCGTTGATA420.12519375223560272No Hit
GTGAATGGGTAGACGTAGCAAAAAAAAAAAAAA390.1162513413616311No Hit
TATCAACGCAGAGTGAATGGGTAGACGTAGAAA380.11327053773697389No Hit
GTGAATGGGATAGACGTAAAAAAAAAAAAAAAA380.11327053773697389No Hit
GCCTAGACGTAGCTCACGTACTCTGCGTTGATA370.11028973411231668No Hit
GATAGACGTAGCTCACGTACTCTGCGTTGATAC370.11028973411231668No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT350.10432812686300226No Hit
TATCAACGCAGAGTGAATGGGTAGACGTAAAAA350.10432812686300226No Hit
GAGTGAATGGGGTAGACGAAAAAAAAAAAAAAA350.10432812686300226No Hit
GAGTGAATGGGTAGACGTAGAAAAAAAAAAAAA340.10134732323834506No Hit
GGATAGACGTAGCTCACGTACTCTGCGTTGATA340.10134732323834506No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCACTG551.3436875E-824.54545427
TACCACT551.3436875E-824.54545426
ATCTACG300.002219242722.5000022
TCTACGC300.002219242722.5000023
TACGCAG300.002219242722.5000025
TATCTAC300.002219242722.5000021
CTACGCA300.002219242722.5000024
ATACCAC657.895869E-820.76923225
GATACCA753.5653284E-718.024
TGATACC851.3216595E-615.88235423
TATCAAC2700.013.51
TCAACGC2900.012.5689653
ATCAACG3000.012.152
CGTTGAT3500.011.57142827
CAACGCA3250.011.21538454
TTGATAC1301.0493442E-410.38461622
GTATCAA3000.010.3499991
GTTGATA1957.091712E-79.69230727
AACGCAG3950.09.2278485
GCGTTGA4300.09.10465126
Produced by FastQC (version 0.11.2)