FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate1_48.3520000005b71a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate1_48.3520000005b71a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46267
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCCACGCTTAACGTACTCTGCGTTGATACCA8721.8847126461624917No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1460.315559686169408No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1440.3112369507424298No Hit
GTGAATGGGGTTCCACAAAAAAAAAAAAAAAAA1260.2723323318996261No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1180.2550413901917133No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1150.24855728705124605No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1140.24639591933775695No Hit
GTGAATGGGTTCCACAAAAAAAAAAAAAAAAAA1070.23126634534333326No Hit
GTTCCACGCAAAAAAAAAAAAAAAAAAAAAAAA1040.224782242202866No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA950.2053299327814641No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA890.19236172650052955No Hit
GTGAATGGGTTCCACGCAAAAAAAAAAAAAAAA880.19020035878704045No Hit
CGCTTAACGTACTCAAAAAAAAAAAAAAAAAAA750.1621025785116822No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA700.1512957399442367No Hit
TTCCACGCTTAACGTACTCTGCGTTGATACCAC640.13832753366330214No Hit
GTGAATGGGGTTCCACGAAAAAAAAAAAAAAAA640.13832753366330214No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA590.12752069509585665No Hit
GAGTGAATGGGTTCCACAAAAAAAAAAAAAAAA590.12752069509585665No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA590.12752069509585665No Hit
CCCCCGTTCCACGCTTAACGTACTCTGCGTTGA540.11671385652841117No Hit
ACGCAGAGTGAATGGGTTCCACAAAAAAAAAAA530.11455248881492208No Hit
GAGTGAATGGGGTTCCACAAAAAAAAAAAAAAA520.112391121101433No Hit
GTGAATGGGTTCCAAAAAAAAAAAAAAAAAAAA510.11022975338794389No Hit
CGTTAAGCGTGGAAAAAAAAAAAAAAAAAAAAA510.11022975338794389No Hit
GTGAATGGGTTCCACGAAAAAAAAAAAAAAAAA510.11022975338794389No Hit
CCCCAGTTCCACGCTTAACGTACTCTGCGTTGA490.1059070179609657No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA480.1037456502474766No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACTTA200.00852651227.022
CTTACTC200.00852651227.025
CTTAACT200.00852651227.020
GTTGATA1150.022.30434824
CGTTGAT1150.022.30434823
GATACCA1200.021.37500227
TGATACC1200.021.37500226
TTGATAC1300.019.7307725
TGGGGCG350.005454445319.28571313
GCGTTGA3150.016.28571327
TATCAAC2850.013.7368421
ATCAACG3100.012.6290332
TGGGTTC1203.4520526E-612.3750016
AATGGGT1801.537046E-912.0000014
TCAACGC3150.011.9999993
TCTGCGT10400.011.68269327
ATGGGTT1308.4341555E-611.4230785
TGCGTTG5400.011.2527
CAACGCA3400.011.1176474
GTATCAA2655.820766E-1110.1886791
Produced by FastQC (version 0.11.2)