FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate1_42.3520000005b65f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate1_42.3520000005b65f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71354
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGTACTCTGCGTTGATACAAAAAAAAAAAAA2490.3489643187487737No Hit
AACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA1970.2760882361185077No Hit
GTGAATGGGCAGTCTCAAAAAAAAAAAAAAAAA1400.19620483785071616No Hit
ACTCTGCGTTGATACAAAAAAAAAAAAAAAAAA1380.1934019115957059No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1360.1905989853406957No Hit
TATCAACGCAGAGTGAATGGGAACGTACTCTGC1290.18078874344815987No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1270.17798581719314965No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1270.17798581719314965No Hit
GTGAATGGGCAGTCTCTGGAAAAAAAAAAAAAA1260.17658435406564454No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1150.16116825966308826No Hit
GTATCAACGCAGAGTGAATGGGAACGTACTCTG1110.15556240715306782No Hit
AACGTACTCTGCGTTGATACCAAAAAAAAAAAA1090.15275948089805758No Hit
GAAACGTACTCTGCGTAAAAAAAAAAAAAAAAA1030.1443507021330269No Hit
TATCAACGCAGAGTGAATGGGAAACGTACTCTG1020.14294923900552176No Hit
GCGTTGATACAAAAAAAAAAAAAAAAAAAAAAA1010.14154777587801665No Hit
GTGAATGGGAACGTACTCTGCGTTGATACAAAA1010.14154777587801665No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1000.14014631275051154No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA900.1261316814754604No Hit
GGAACGTACTCTGCGTTGATACAAAAAAAAAAA890.12473021834795525No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA890.12473021834795525No Hit
GTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAA880.12332875522045016No Hit
GAGTACGTTTCCAAAAAAAAAAAAAAAAAAAAA870.12192729209294503No Hit
GTATCAACGCAGAGTGAATGGGAAACGTACTCT820.11491997645541946No Hit
GTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA790.1107155870729041No Hit
ACGCAGAGTGAATGGGAACGTACTCTGCGTTGA780.109314123945399No Hit
GCGTTGATACCAAAAAAAAAAAAAAAAAAAAAA760.10651119769038878No Hit
TATCAACGCAGAGTGAATGGGCAGTCTCTGGAA720.1009053451803683No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTGA307.082578E-526.990891
ATCTACG307.082578E-526.990892
TCTACGC307.082578E-526.990893
TACGCAG257.770959E-426.990895
ATCATCG604.3655746E-1126.990893
CTACGCA307.082578E-526.990894
TCATCGC554.7293724E-1026.990894
CATCGCA554.7293724E-1026.990895
CTGAGTG200.008571397526.9908894
GTTTCAA200.008571397526.9908891
TAACCCA200.008571397526.9908894
GATCAGT200.008571397526.9908896
TGAGTGA200.008571397526.9908895
TATCATC651.200533E-1024.9146672
GTATCAT601.2969394E-924.741651
ATCGCAG601.2969394E-924.741656
TCGCAGA551.4048055E-824.5371727
TATCTAC352.0176891E-423.135051
AACCCAG300.002248418822.4924095
CGCTGAG404.9755414E-420.2431662
Produced by FastQC (version 0.11.2)