FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate1_41.3520000005b635.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate1_41.3520000005b635.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55691
Sequences flagged as poor quality0
Sequence length33
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATCGGTTGAACGTACTCTGCGTTGATACCA4480.8044387782586055No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1970.3537375877610386No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1860.3339857427591532No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1830.32859887594045717No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1720.30884703093857174No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1640.29448205275538236No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA1390.24959149593291557No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA1350.24240900684132086No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA1050.18854033865436068No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA930.1669928713795766No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA890.1598103822879819No Hit
CCCTATCGGTTGAACGTACTCTGCGTTGATACC870.15621913774218457No Hit
GTGAATGGGCCTATCGGAAAAAAAAAAAAAAAA680.12210231455710976No Hit
GAGTACGTTCAAAAAAAAAAAAAAAAAAAAAAA650.11671544773841375No Hit
GTGAATGGGCCTATCGGTAAAAAAAAAAAAAAA630.11312420319261639No Hit
GAGTACGTTCAACCAAAAAAAAAAAAAAAAAAA580.10414609182812302No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCT356.4623327E-626.9856265
AGCCCTA455.4677002E-826.9856246
AAGCCCT200.00856360226.9856225
GATACCA601.2714736E-924.75906827
TCTGCGT17300.020.68676827
TGTCCTA608.786392E-720.2392186
ATACCTA559.443818E-619.6259086
AACTCCT350.00548519319.2754485
TAACCTA651.8922528E-618.6823566
ATGGGAG552.1092857E-417.172675
TGATACC951.3291356E-817.05887826
TTGATAC1054.562753E-815.43422425
ACACCTA707.496075E-515.4203586
GCACCTA707.496075E-515.4203586
AACCTAT1850.015.3161657
GAACCTA550.0041888214.7194316
ATTCCTA550.0041888214.7194316
CTTCCTA550.0041888214.7194316
TCTCCTA657.5208885E-414.5307216
CTACCTA657.5208885E-414.5307217
Produced by FastQC (version 0.11.2)