FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate1_4.3520000005b21f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate1_4.3520000005b21f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90259
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCGACGTCTACGTACTCTGCGTTGATACCA12181.3494499163518319No Hit
GTGAATGGGTATCGACGTCAAAAAAAAAAAAAA6360.7046388725778039No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA5100.5650406053689936No Hit
GTGAATGGGGTATCGACGTCAAAAAAAAAAAAA4940.547313841278986No Hit
GTGAATGGGTATCGACGTAAAAAAAAAAAAAAA4930.5462059185233605No Hit
GTGAATGGGTATCGACGAAAAAAAAAAAAAAAA4090.4531404070508204No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3080.34124020873264715No Hit
TATCAACGCAGAGTGAATGGGTATCGACGTCTA3030.33570059495451976No Hit
GTGAATGGGTATCGACGTCTAAAAAAAAAAAAA2660.2947074529963771No Hit
GTGAATGGGGTATCGACGTAAAAAAAAAAAAAA2630.29138368472950066No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA2160.23931131521510318No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA2150.23820339245947775No Hit
GTGAATGGGGTATCGACGAAAAAAAAAAAAAAA2110.2337717014369758No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA2040.22601624214759747No Hit
GTATCAACGCAGAGTGAATGGGTATCGACGTCT2020.22380039663634652No Hit
ACGCAGAGTGAATGGGTATCGACGTCAAAAAAA1990.2204766283694701No Hit
GTGAATGGGTATCGACGTCTACAAAAAAAAAAA1970.21826078285821912No Hit
GTATCGACGTCAAAAAAAAAAAAAAAAAAAAAA1960.21715286010259363No Hit
GTGAATGGGTATCGACGTCTACGTACTCAAAAA1910.21161324632446624No Hit
ACGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1850.2049657097907134No Hit
ACGCAGAGTGAATGGGTATCGACGTAAAAAAAA1840.20385778703508792No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1830.20274986427946243No Hit
ACGCAGAGTGAATGGGTATCGACGAAAAAAAAA1670.18502310018945478No Hit
TATCAACGCAGAGTGAATGGGTATCGACGTCAA1630.18059140916695288No Hit
ACGCAGAGTGAATGGGTATCGACGTCTACGTAC1620.1794834864113274No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA1520.16840425885507262No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA1430.15843295405444333No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1370.15178541752069047No Hit
GTATCGACGTAAAAAAAAAAAAAAAAAAAAAAA1340.14846164925381403No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1340.14846164925381403No Hit
GTGAATGGGGTATCGACAAAAAAAAAAAAAAAA1260.1395982672088102No Hit
TATCAACGCAGAGTGAATGGGTATCGACGTAAA1220.13516657618630828No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA1210.13405865343068282No Hit
GTGAATGGGTATCGACGTCTACGTACAAAAAAA1210.13405865343068282No Hit
TATCAACGCAGAGTGAATGGGTATCGACGAAAA1190.13184280791943187No Hit
GTGAATGGGTATCGACGTCTACGAAAAAAAAAA1110.12297942587442803No Hit
AACGCAGAGTGAATGGGTATCGACGTCTACGTA1090.12076358036317708No Hit
GAGTGAATGGGTATCGACGTCAAAAAAAAAAAA1060.11743981209630064No Hit
GAGTGAATGGGGTATCGACGTCAAAAAAAAAAA1050.11633188934067518No Hit
GAGTGAATGGGTATCGACGAAAAAAAAAAAAAA1040.11522396658504969No Hit
GTGAATGGGTATCGACAAAAAAAAAAAAAAAAA1030.11411604382942421No Hit
GTATCGACGTCTACGTACTCAAAAAAAAAAAAA1030.11411604382942421No Hit
TATCAACGCAGAGTGAATGGGGTATCGACGTCA1020.11300812107379873No Hit
AACGCAGAGTGAATGGGTATCGACGTCAAAAAA1020.11300812107379873No Hit
GTGAATGGGTATCGACGTCTACGTAAAAAAAAA1010.11190019831817326No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA980.10857643005129682No Hit
GAGTGAATGGGTATCGACGTAAAAAAAAAAAAA970.10746850729567135No Hit
ACGCAGAGTGAATGGGGTATCGACGTCAAAAAA960.10636058454004586No Hit
GTATCAACGCAGAGTGAATGGGTATCGACGTCA960.10636058454004586No Hit
GTATCAACGCAGAGTGAATGGGGTATCGACGTC940.10414473902879491No Hit
AACGCAGAGTGAATGGGTATCGACGTAAAAAAA930.10303681627316942No Hit
GGGTATCGACGTCAAAAAAAAAAAAAAAAAAAA920.10192889351754396No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTAAC200.00856304427.0038832
CAACTCA505.135007E-926.9889091
GTATTAA200.00858627126.9889091
GGTATCT352.0168471E-423.14618920
GTATCAT857.2759576E-1222.2261581
TATCATC806.730261E-1121.9406552
TACGCAG454.5374414E-521.0030235
TGATACC2500.019.98287426
GATACCA2450.019.85060527
TCAGAGT751.7109414E-819.8028495
CGCTGAG701.8067112E-719.288492
CTGAGTG701.8067112E-719.288494
AACTCAG701.8067112E-719.288492
ACTCAGA701.8067112E-719.288493
CTCAGAG701.8067112E-719.288494
ACGCTGA701.817316E-719.2777921
AACGTAG803.6110578E-818.5548741
GCTGAGT753.7314567E-718.002593
TAGAGTG906.9958332E-918.002595
TTGATAC2800.017.84185425
Produced by FastQC (version 0.11.2)