FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate1_35.3520000005b56c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate1_35.3520000005b56c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185317
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTTCTGTGACGTACTCTGCGTTGATACCAC19271.039839842000464No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA11350.6124640480905691No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7730.4171230917832686No Hit
GTCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAA7010.37827074688236917No Hit
GTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5610.30272452068617556No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA3920.21152943334934193No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA3900.21045020154653918No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA3410.18400902237787142No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA3220.17375632025124516No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA2960.15972630681480923No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2820.15217168419518987No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT2630.1419189820685636No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA2520.13598320715314838No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA2250.12141357781531106No Hit
CCCCCAGTCCTTCTGTGACGTACTCTGCGTTGA2210.11925511420970553No Hit
CAGTCCTTCTGTGACGTACTCTGCGTTGATACC2150.11601741880129723No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA2050.11062125978728342No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT1200.027.0021611
GTATTAA257.7992096E-427.0021611
ACCCCCG200.00858868527.0021611
CCGCCTC200.00860001226.9948672
ATTAACG257.811533E-426.9948673
TATTAAC257.811533E-426.9948672
CCCCTCT200.00860001226.9948672
TTAACGC257.811533E-426.9948674
CCCCACC200.00860001226.9948672
TATCATC1250.025.9150732
TTCGTTG501.54394E-724.30851225
CTTCGTT501.54394E-724.30851224
CAACTCA1000.024.3019451
TAACGCA401.8454948E-523.620515
GTATCTA352.0289465E-423.1447091
ATCATCG1400.023.1384583
ATACCAC3300.022.91711627
GTAAATG300.0022599922.4957263
TGTGACT553.8727921E-722.09864620
TGACTTA553.8727921E-722.09864622
Produced by FastQC (version 0.11.2)