FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate1_33.3520000005b529.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate1_33.3520000005b529.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73092
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA2920.39949652492748866No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA2640.36118863897553766No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA2460.3365621408635692No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT2380.3256170305915832No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA2220.3037268100476112No Hit
GCCATTCCGTTACGTACTCTGCGTTGATACCAC2210.302358671263613No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA2170.29688611612762No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA1690.23121545449570402No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA1670.22847917692770756No Hit
CAGCCATTCCGTTACGTACTCTGCGTTGATACC1620.2216384830077163No Hit
GAGCCATTCCGTTACGTACTCTGCGTTGATACC1440.19701198489574784No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA1380.18880315219175833No Hit
AGCCATTCCGTTACGTACTCTGCGTTGATACCA1370.1874350134077601No Hit
CCATTCCGTTACGTACTCTGCGTTGATACCACT1300.17785804191977234No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1160.15870409894379686No Hit
GTGAATGGGGAGTACGTAACGAAAAAAAAAAAA1130.1545996825918021No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA940.1286050456958354No Hit
GTACGTAACGAAAAAAAAAAAAAAAAAAAAAAA840.11492365785585289No Hit
CCCCCAGCCATTCCGTTACGTACTCTGCGTTGA830.11355551907185465No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA770.10534668636786515No Hit
GAGTACGTAACGAAAAAAAAAAAAAAAAAAAAA760.10397854758386692No Hit
GTTTAAGCATATCAAAAAAAAAAAAAAAAAAAA760.10397854758386692No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGGCT401.8233464E-523.6244018
GGGTGGC401.8233464E-523.6244017
CCGCAGC300.002244824822.499435
TGGGTGG504.150479E-621.5994516
TGGGAGA559.468766E-619.6358646
GCGTTGA5650.019.60586227
ATGGGTG602.0047297E-517.9995445
GCTAGCC450.00109692117.9995446
TTTAGCC552.1124803E-417.1813813
GAGAGTA653.9864404E-516.6149649
AATGGGT757.4336713E-616.1995894
GGAGAGT707.514893E-515.428188
GGGAGAG707.514893E-515.428187
ATGGGAG1559.094947E-1214.8060765
TATCAAC2350.013.7868841
CGCAGCC600.007463590213.4996586
GGGGAGT700.001318954413.4996587
TGCGTTG10650.012.93811727
CCAAGCC853.907199E-412.7055594
TGATACC1153.1059943E-511.74688427
Produced by FastQC (version 0.11.2)