FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate1_30.3520000005b4ca.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate1_30.3520000005b4ca.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50169
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCGTCACACTACGTACTCTGCGTTGATACCA3540.7056150212282486No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA3010.5999720943211944No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA2580.5142617951324523No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT2500.49831569295780265No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA2500.49831569295780265No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA2380.47439653969582807No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA2180.4345312842592039No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA2060.4106121309972293No Hit
GTGAATGGGTTCGTCAAAAAAAAAAAAAAAAAA1940.38669297773525485No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA1540.3069624668620064No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA1450.2890231019155255No Hit
GTGAATGGGGTTCGTCAAAAAAAAAAAAAAAAA1440.2870298391436943No Hit
GTGAATGGGTTCGTCACAAAAAAAAAAAAAAAA1400.27905678805636946No Hit
CACTACGTACTCAAAAAAAAAAAAAAAAAAAAA850.1694273356056529No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA850.1694273356056529No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT810.16145428451832805No Hit
GTGAATGGGTTCGTCACACAAAAAAAAAAAAAA770.1534812334310032No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA770.1534812334310032No Hit
TATCAACGCAGAGTGAATGGGTTCGTCACACTA680.13554186848452232No Hit
GTGAATGGGTTCGTCACACTACGTACTCAAAAA650.12956208016902868No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA600.11959576630987262No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA580.11560924076621021No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA570.113615977994379No Hit
GTCACACTACGTACTCAAAAAAAAAAAAAAAAA530.10564292690705417No Hit
ATCAACGCAGAGTGAATGGGAAGCAGTGGTATC530.10564292690705417No Hit
GAATGGGAAGCAGTGGTATCAAAAAAAAAAAAA530.10564292690705417No Hit
CTACGTACTCAAAAAAAAAAAAAAAAAAAAAAA520.10364966413522293No Hit
CCCCCCGTTCGTCACACTACGTACTCTGCGTTG520.10364966413522293No Hit
ACGCAGAGTGAATGGGTTCGTCAAAAAAAAAAA510.10165640136339173No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAGTG257.7231927E-427.04
ACGCTGA257.7231927E-427.01
CACCGTT257.7231927E-427.05
TGAGTGA257.7231927E-427.05
CGCTGAG352.0014349E-423.1428592
GCTGAGT300.002234744122.53
CCCCCGT300.002234744122.54
CCCCCAG300.002234744122.52
AACGTTC501.00985264E-418.9000026
GCTGTTC450.001090209718.0000026
GGTGTTC450.001090209718.0000026
TCTGCGT13350.017.69662927
TGCGTTG3200.015.60937527
ACGTTCG2000.015.5250017
TGGGAGT550.004166266414.7272736
CACGTTC904.113271E-513.5000016
CTCTGCG17500.013.526
ACCGTTC700.001308617113.56
TATCAAC2700.013.51
ACTCTGC19100.012.3691125
Produced by FastQC (version 0.11.2)