FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate1_29.3520000005b4a1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate1_29.3520000005b4a1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66852
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTGGACCTTACGTACTCTGCGTTGATACCA8841.3223239394483337No Hit
GCTTGGACCTTACGTACTCTGCGTTGATACCAC7821.1697481002812182No Hit
GCCTTACGTACTCAAAAAAAAAAAAAAAAAAAA5050.7553999880332676No Hit
GGCCTTACGTACTCAAAAAAAAAAAAAAAAAAA2480.37096870699455514No Hit
TATCAACGCAGAGTGAATGGGCCTTACGTACTC2410.3604978160713217No Hit
GTACGTAAGGTCCAAAAAAAAAAAAAAAAAAAA2100.31412672769700234No Hit
GACCTTACGTACTCAAAAAAAAAAAAAAAAAAA1850.2767306886854544No Hit
GTGAATGGGAGGTCCAAAAAAAAAAAAAAAAAA1670.24980554059713997No Hit
GTGAATGGGCCTTACGTACTCAAAAAAAAAAAA1630.24382217435529227No Hit
CCTTACGTACTCAAAAAAAAAAAAAAAAAAAAA1620.24232633279483037No Hit
ACGCAGAGTGAATGGGCCTTACGTACTCAAAAA1600.23933464967390655No Hit
GTATCAACGCAGAGTGAATGGGCCTTACGTACT1570.2348471249925208No Hit
GAGTACGTAAGGTCCAAAAAAAAAAAAAAAAAA1500.2243762340692874No Hit
GATACCACTGCTTGGACCTTACGTACTCTGCGT1450.2168970262669778No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1320.1974510859809729No Hit
GAATGGGACCTTACGTACTCAAAAAAAAAAAAA1320.1974510859809729No Hit
GTGAATGGGACCTTACGTACTCAAAAAAAAAAA1270.18997187817866332No Hit
TACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA1250.1869801950577395No Hit
GACCTAAAAAAAAAAAAAAAAAAAAAAAAAAAA1230.18398851193681565No Hit
ACCTTACGTACTCAAAAAAAAAAAAAAAAAAAA1200.1795009872554299No Hit
GTGAATGGGGAGTACGTAAGGTCCAAAAAAAAA1150.17202177945312033No Hit
GTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1140.1705259378926584No Hit
GTGAATGGGTGCTTGGACCAAAAAAAAAAAAAA1100.16454257165081074No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1040.15556752228803927No Hit
GTGCTTGGACCTTACGTACTCTGCGTTGATACC1000.1495841560461916No Hit
GTGAATGGGTACGTAAGGTCCAAAAAAAAAAAA1000.1495841560461916No Hit
GAGTGAATGGGCCTTACGTACTCAAAAAAAAAA970.14509663136480583No Hit
GAATGGGCCTTACGTACTCAAAAAAAAAAAAAA940.14060910668342008No Hit
GGTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA930.13911326512295819No Hit
GGACCTTACGTACTCAAAAAAAAAAAAAAAAAA920.13761742356249626No Hit
GCTTGGACCTTACGTACTCTGCGTTTATACCAC880.1316340573206486No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT880.1316340573206486No Hit
CTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA840.12565069107880095No Hit
ACGCAGAGTGAATGGGACCTTACGTACTCAAAA830.12415484951833902No Hit
TATCAACGCAGAGTGAATGGGACCTTACGTACT810.12116316639741519No Hit
GTGAATGGGAGTACGTAAGGTCCAAAAAAAAAA810.12116316639741519No Hit
GTGAATGGGGTACGTAAGGTCCAAAAAAAAAAA770.11517980015556752No Hit
TACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA770.11517980015556752No Hit
GTATCAACGCAGAGTGAATGGGACCTTACGTAC760.1136839585951056No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA760.1136839585951056No Hit
AACGCAGAGTGAATGGGCCTTACGTACTCAAAA760.1136839585951056No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA750.1121881170346437No Hit
GGTACGTAAGGTCCAAAAAAAAAAAAAAAAAAA750.1121881170346437No Hit
GCGTTGATACCACTGCTTGGACCTTACGTACTC710.10620475079279604No Hit
TACGTACTCTGCGTTGATACAAAAAAAAAAAAA710.10620475079279604No Hit
ACGCAGAGTGAATGGGCCTTACGTACTCTGCGT700.10470890923233413No Hit
GCTTGGACCAAAAAAAAAAAAAAAAAAAAAAAA680.10171722611141028No Hit
GTGAATGGGGGTCCAAAAAAAAAAAAAAAAAAA680.10171722611141028No Hit
GGGTACGTAAGGTCCAAAAAAAAAAAAAAAAAA680.10171722611141028No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACTCT200.00852338127.01947826
CAACTCA200.00852338127.0194781
AACTCAG200.00852338127.0194782
CTTACTC200.00852338127.01947825
GTATCAT307.02503E-527.0194761
TATCATC307.02503E-527.0194762
ACTCAGA200.00855461226.9992523
CTCAGAG200.00858593326.979064
TTGTGCT307.097141E-526.9790576
ACGCTGA501.5079058E-724.317531
ATCATCG401.8203858E-523.6243463
ATCTGCT352.02183E-423.1249077
CGCTGAG553.7830068E-722.1068442
CTGAGTG553.8381404E-722.0737744
TGAGTGA553.8381404E-722.0737745
TCGCAGA454.5470453E-520.9837117
ATCGCAG454.5470453E-520.9837116
TCATCGC454.5470453E-520.9837114
CATCGCA454.5470453E-520.9837115
GCTGAGT608.7982335E-720.249443
Produced by FastQC (version 0.11.2)