FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate1_28.3520000005b487.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate1_28.3520000005b487.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65354
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTCCTCTACGTACTCTGCGTTGATACCA6701.0251859105793066No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA2610.399363466658506No Hit
CTCGTCCTCTACGTACTCTGCGTTGATACCACT2580.39477308198427025No Hit
GTGAATGGGATCTCGTCCAAAAAAAAAAAAAAA2270.347339107017168No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA2190.3350980812192062No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA2150.32897756832022523No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA2110.32285705542124427No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1890.28919423447684917No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA1710.2616519264314349No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA1550.2371698748355112No Hit
GAGTGAATGGGATCTCGTCCAAAAAAAAAAAAA1520.23257949016127552No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA1390.21268782323958746No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA1280.1958564127673899No Hit
GTGAATGGGATCTCGTCAAAAAAAAAAAAAAAA1230.18820577164366373No Hit
TATCAACGCAGAGTGAATGGGATCTCGTCCAAA1150.17596474584570188No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1150.17596474584570188No Hit
ACGCAGAGTGAATGGGATCTCGTCCAAAAAAAA1060.16219359182299478No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA1030.15760320714875906No Hit
CATCTCGTCCTCTACGTACTCTGCGTTGATACC980.1499525660250329No Hit
GTGAATGGGATCTCGTCCTCAAAAAAAAAAAAA840.12853077087859963No Hit
GAGTGAATGGGATCTCGTCAAAAAAAAAAAAAA700.10710897573216636No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAGTG200.0085521227.04
AGTATCT200.0085521227.06
ATACCAC553.8068356E-722.0909126
TACCACT608.789866E-720.2527
AATGGGT701.7904676E-719.2857154
GATACCA1700.015.88235427
TGGGGAT707.494074E-515.4285726
TATCAAC4200.015.4285711
TGATACC1800.015.026
ATCAACG4350.014.5862062
GCGTTGA4900.014.05102127
TCAACGC4700.013.5000013
TTGATAC2000.013.525
CCGATCT700.00131612413.56
GTTGATA2050.013.17073224
CAACGCA5150.012.3203894
GGGGATC800.003589077211.81257
CGTTGAT2409.094947E-1211.2523
TGCGTTG8200.011.03048727
TCTGCGT17000.010.95882427
Produced by FastQC (version 0.11.2)