FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate1_24.3520000005b400.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate1_24.3520000005b400.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51431
Sequences flagged as poor quality0
Sequence length33
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCACACTACACACGTACTCTGCGTTGATAC5090.9896754875464214No Hit
CCACACTACACACGTACTCTGCGTTGATACCAC3510.6824677723551944No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1880.3655382940250044No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1770.34415041511928607No Hit
GCTCCACACTACACACGTACTCTGCGTTGATAC1740.3383173572359083No Hit
ACTCCACACTACACACGTACTCTGCGTTGATAC1600.31109642044681224No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA1530.29748595205226425No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1470.2858198362855087No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1360.2644319573797904No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1300.25276584161303495No Hit
CTCCACACTACACACGTACTCTGCGTTGATACC1230.2391553732184869No Hit
CTTCCACACTACACACGTACTCTGCGTTGATAC1180.22943361007952404No Hit
CGTGTGTAGTGTGAAAAAAAAAAAAAAAAAAAA1160.22554490482393888No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA1130.21971184694056112No Hit
CATCCACACTACACACGTACTCTGCGTTGATAC1130.21971184694056112No Hit
CGTCCACACTACACACGTACTCTGCGTTGATAC1080.20999008380159825No Hit
GTGTAGTGTGGAAAAAAAAAAAAAAAAAAAAAA1060.2061013785460131No Hit
GGTGTGTAGTGTGGAAAAAAAAAAAAAAAAAAA1050.20415702591822052No Hit
TCTCCACACTACACACGTACTCTGCGTTGATAC1040.20221267329042794No Hit
GTCCACACTACACACGTACTCTGCGTTGATACC1020.1983239680348428No Hit
CCCCTCCACACTACACACGTACTCTGCGTTGAT1000.19443526277925766No Hit
CGTGTGTAGTGTGGAAAAAAAAAAAAAAAAAAA990.19249091015146508No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA980.1905465575236725No Hit
GGTCCACACTACACACGTACTCTGCGTTGATAC830.16138126810678385No Hit
GGTGTGTAGTGTGAAAAAAAAAAAAAAAAAAAA820.15943691547899128No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA720.13999338920106552No Hit
ATTCCACACTACACACGTACTCTGCGTTGATAC670.13027162606210263No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA630.12249421555093232No Hit
CTACACACGTACTCAAAAAAAAAAAAAAAAAAA630.12249421555093232No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA610.11860551029534716No Hit
GTGAATGGGGTGTGTAGTGTGGAAAAAAAAAAA600.1166611576675546No Hit
GATCCACACTACACACGTACTCTGCGTTGATAC590.11471680503976202No Hit
GTTCCACACTACACACGTACTCTGCGTTGATAC570.11082809978417685No Hit
GTGAATGGGGTGTGTAGTGTGAAAAAAAAAAAA520.10110633664521397No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACCA504.109994E-621.59999826
ATACCAC608.714451E-720.2527
TGCCTCC350.0054626819.2857155
GCTTCCA501.01048034E-418.96
GTCTCCA450.001090715618.0000026
TTGATAC2400.018.027
GTTCCAC807.1985596E-716.8757
ATGGGAC550.00416815614.7272735
AGCTCCA550.00416815614.7272737
GTTGATA2800.014.46428626
TGATACC751.340276E-414.425
TCTGCGT17950.013.76323127
TTTCCAC600.00742275813.57
TCTCCAC2350.012.6382987
TATCAAC2550.012.176471
CTCTCCA800.003571098211.81256
CGCTCCA800.003571098211.81256
TGGGGTG1401.5145451E-611.5714296
TATCCAC1308.486417E-611.4230787
GCCTCCA1351.2906405E-511.06
Produced by FastQC (version 0.11.2)