FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate1_20.3520000005b388.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate1_20.3520000005b388.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52150
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTAGGAGACTACGTACTCTGCGTTGATACCA7101.361457334611697No Hit
GTAGGAGACTACGTACTCTGCGTTGATACCACT6661.277085330776606No Hit
GTGAATGGGACGTAGGAGACAAAAAAAAAAAAA2950.5656759348034516No Hit
ACGTAGGAGAAAAAAAAAAAAAAAAAAAAAAAA2890.5541706615532118No Hit
ACGTAGGAGACAAAAAAAAAAAAAAAAAAAAAA2600.49856184084372007No Hit
GTGAATGGGACGTAGGAGAAAAAAAAAAAAAAA2440.4678811121764142No Hit
GGAGACAAAAAAAAAAAAAAAAAAAAAAAAAAA2080.39884947267497606No Hit
GGACGTAGGAGACAAAAAAAAAAAAAAAAAAAA1720.3298178331735379No Hit
ACGCAGAGTGAATGGGACGTAGGAGACAAAAAA1690.32406519654841803No Hit
TATCAACGCAGAGTGAATGGGACGTAGGAGACA1540.29530201342281875No Hit
ACGCAGAGTGAATGGGACGTAGGAGAAAAAAAA1500.28763183125599234No Hit
GGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1460.2799616490891659No Hit
CGTAGGAGACTACGTACTCTGCGTTGATACCAC1450.2780441035474593No Hit
TATCAACGCAGAGTGAATGGGACGTAGGAGAAA1270.24352828379674016No Hit
GAGTGAATGGGACGTAGGAGACAAAAAAAAAAA1270.24352828379674016No Hit
GGACGTAGGAGAAAAAAAAAAAAAAAAAAAAAA1210.23202301054650049No Hit
GAGTGAATGGGACGTAGGAGAAAAAAAAAAAAA1140.21860019175455417No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1130.21668264621284755No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1040.19942473633748803No Hit
GTGAATGGGGACGTAGGAGAAAAAAAAAAAAAA970.1860019175455417No Hit
GTAGGAGACAAAAAAAAAAAAAAAAAAAAAAAA950.18216682646212848No Hit
GTGAATGGGACGTAGGAAAAAAAAAAAAAAAAA910.174496644295302No Hit
GAATGGGACGTAGGAGACAAAAAAAAAAAAAAA900.1725790987535954No Hit
AACGCAGAGTGAATGGGACGTAGGAGACAAAAA900.1725790987535954No Hit
GAATGGGACGTAGGAGAAAAAAAAAAAAAAAAA830.15915627996164908No Hit
GTGAATGGGGACGTAGGAGACAAAAAAAAAAAA800.15340364333652923No Hit
GTATCAACGCAGAGTGAATGGGACGTAGGAGAC710.1361457334611697No Hit
GACGTAGGAGACAAAAAAAAAAAAAAAAAAAAA690.13231064237775647No Hit
AACGCAGAGTGAATGGGACGTAGGAGAAAAAAA690.13231064237775647No Hit
GAACGTAGGAGAAAAAAAAAAAAAAAAAAAAAA690.13231064237775647No Hit
GAACGTAGGAGACAAAAAAAAAAAAAAAAAAAA680.13039309683604985No Hit
GACGTAGGAGAAAAAAAAAAAAAAAAAAAAAAA670.12847555129434324No Hit
GTATCAACGCAGAGTGAATGGGACGTAGGAGAA630.12080536912751677No Hit
GTGAATGGGAACGTAGGAGACAAAAAAAAAAAA600.11505273250239692No Hit
ACGCAGAGTGAATGGGACGTAGGAAAAAAAAAA600.11505273250239692No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA580.1112176414189837No Hit
CCACGTAGGAGACTACGTACTCTGCGTTGATAC580.1112176414189837No Hit
CACGTAGGAGACTACGTACTCTGCGTTGATACC580.1112176414189837No Hit
GTGAATGGGAACGTAGGAGAAAAAAAAAAAAAA580.1112176414189837No Hit
GAGACAAAAAAAAAAAAAAAAAAAAAAAAAAAA550.10546500479386386No Hit
GTATCATCGCAGAGTGAATGGGACGTAGGAGAC530.10162991371045062No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACTCA850.027.0153711
GTATCAT455.3953045E-827.0153711
GAGTAAA200.00851272427.015371
TATCATC455.4460543E-826.9894452
ATGGGTC200.00855275926.9894435
CGCTGAG200.00855275926.9894432
TAAATGG200.00855275926.9894434
AGTAAAT200.00855275926.9894432
AAATGGG200.00855275926.9894435
GTAAATG200.00855275926.9894433
TACCACT801.8189894E-1223.63844727
AACTCAG950.022.7279552
ACTCAGA950.022.7279553
CTCAGAG950.022.7279554
TCAGAGT900.022.4912035
TATCTAC454.4755267E-521.0119551
ATACCAC1006.366463E-1118.91075926
ATCATCG651.8849569E-618.6853
TCATCGC651.8849569E-618.6854
CATCGCA651.8849569E-618.6855
Produced by FastQC (version 0.11.2)