FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate1_16.3520000005b31e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate1_16.3520000005b31e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34748
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTATGGTGTTCACGTACTCTGCGTTGATACCA6571.8907563025210083No Hit
GTGAATGGGCTATGGTGAAAAAAAAAAAAAAAA5071.4590767813974905No Hit
CCGCTATGGTGTTCACGTACTCTGCGTTGATAC3150.9065269943593876No Hit
GTGAATGGGGCTATGGTGAAAAAAAAAAAAAAA2620.754000230229078No Hit
ACGCAGAGTGAATGGGCTATGGTGAAAAAAAAA2320.6676643260043744No Hit
GTGAATGGGCTATGGTGTAAAAAAAAAAAAAAA2140.6158627834695521No Hit
GGGCTATGGTGTTCACGTACTCTGCGTTGATAC2080.5985956026246115No Hit
GGGCTATGGTGAAAAAAAAAAAAAAAAAAAAAA2030.5842062852538276No Hit
TATCAACGCAGAGTGAATGGGCTATGGTGAAAA1680.4834810636583401No Hit
GCGCTATGGTGTTCACGTACTCTGCGTTGATAC1460.42016806722689076No Hit
GAGTGAATGGGCTATGGTGAAAAAAAAAAAAAA1420.4086566133302636No Hit
GGTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAA1350.3885115690111661No Hit
GTGTTCACGTACTCAAAAAAAAAAAAAAAAAAA1210.3482214803729711No Hit
GAATGGGCTATGGTGAAAAAAAAAAAAAAAAAA1190.3424657534246575No Hit
CTGCTATGGTGTTCACGTACTCTGCGTTGATAC1170.33671002647634396No Hit
CTATGGTGTTCACGTACTCTGCGTTGATACCAC1080.3108092552089329No Hit
ACGCAGAGTGAATGGGCTATGGTGTAAAAAAAA1070.30793139173477607No Hit
GGCTATGGTGTTCACGTACTCTGCGTTGATACC1060.3050535282606193No Hit
GTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1030.29641993783814896No Hit
GTGAATGGGCTATGGTAAAAAAAAAAAAAAAAA1010.2906642108898354No Hit
AACGCAGAGTGAATGGGCTATGGTGAAAAAAAA1000.2877863474156786No Hit
GCTATGGTGAAAAAAAAAAAAAAAAAAAAAAAA970.27915275699320824No Hit
GTATCAACGCAGAGTGAATGGGCTATGGTGAAA970.27915275699320824No Hit
GAATGGGGCTATGGTGAAAAAAAAAAAAAAAAA910.2618855761482675No Hit
GTGCTATGGTGTTCACGTACTCTGCGTTGATAC890.25612984919995396No Hit
CGGCTATGGTGTTCACGTACTCTGCGTTGATAC880.25325198572579716No Hit
ACGCTATGGTGTTCACGTACTCTGCGTTGATAC880.25325198572579716No Hit
GAGTGAATGGGGCTATGGTGAAAAAAAAAAAAA870.25037412225164035No Hit
TCGCTATGGTGTTCACGTACTCTGCGTTGATAC860.2474962587774836No Hit
GGCTATGGTGAAAAAAAAAAAAAAAAAAAAAAA850.2446183953033268No Hit
GTGAATGGGGCTATGGTAAAAAAAAAAAAAAAA820.23598480488085646No Hit
GTGAATGGGCTATGGAAAAAAAAAAAAAAAAAA810.23310694140669966No Hit
CAGCTATGGTGTTCACGTACTCTGCGTTGATAC810.23310694140669966No Hit
GGGCTATGGTGTAAAAAAAAAAAAAAAAAAAAA800.23022907793254285No Hit
GAGCTATGGTGTTCACGTACTCTGCGTTGATAC770.22159548751007255No Hit
ATGCTATGGTGTTCACGTACTCTGCGTTGATAC770.22159548751007255No Hit
GTGAATGGGGCTATGGAAAAAAAAAAAAAAAAA740.21296189708760216No Hit
GGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA680.19569471624266144No Hit
TATCAACGCAGAGTGAATGGGCTATGGTGTAAA670.19281685276850466No Hit
GCTATGGTGTAAAAAAAAAAAAAAAAAAAAAAA640.1841832623460343No Hit
CGCTATGGTGTTCACGTACTCTGCGTTGATACC630.18130539887187752No Hit
ACGCAGAGTGAATGGGGCTATGGTGAAAAAAAA620.17842753539772072No Hit
GAGTGAATGGGCTATGGTGTAAAAAAAAAAAAA590.16979394497525038No Hit
ATCAACGCAGAGTGAATGGGCTATGGTGAAAAA580.1669160815010936No Hit
TTGCTATGGTGTTCACGTACTCTGCGTTGATAC560.16116035455278002No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT550.15828249107862322No Hit
ACGCAGAGTGAATGGGCTATGGTAAAAAAAAAA550.15828249107862322No Hit
GTGAATGGGGCTATGGTGTAAAAAAAAAAAAAA540.15540462760446644No Hit
GTATCATCGCAGAGTGAATGGGCTATGGTGAAA530.15252676413030966No Hit
TATCAACGCAGAGTGAATGGGGCTATGGTGAAA520.14964890065615288No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA520.14964890065615288No Hit
AAGCTATGGTGTTCACGTACTCTGCGTTGATAC520.14964890065615288No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA500.1438931737078393No Hit
ACGCAGAGTGAATGGGCTATGGAAAAAAAAAAA480.13813744675952574No Hit
AACGCAGAGTGAATGGGCTATGGTGTAAAAAAA460.13238171981121213No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA460.13238171981121213No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA450.12950385633705536No Hit
GGCTATGGTAAAAAAAAAAAAAAAAAAAAAAAA450.12950385633705536No Hit
TATCTACGCAGAGTGAATGGGCTATGGTGAAAA430.1237481293887418No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA430.1237481293887418No Hit
GTATCAACGCAGAGTGAATGGGCTATGGTGTAA430.1237481293887418No Hit
TAGCTATGGTGTTCACGTACTCTGCGTTGATAC400.11511453896627143No Hit
GAATGGGCTATGGTGTAAAAAAAAAAAAAAAAA390.11223667549211465No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA390.11223667549211465No Hit
CAACGCAGAGTGAATGGGCTATGGTGAAAAAAA390.11223667549211465No Hit
TATCAACGCAGAGTGAATGGGCTATGGTAAAAA380.10935881201795787No Hit
AGGCTATGGTGTTCACGTACTCTGCGTTGATAC370.10648094854380108No Hit
GAATGGGGGCTATGGAAAAAAAAAAAAAAAAAA370.10648094854380108No Hit
AACGCAGAGTGAATGGGGCTATGGTGAAAAAAA350.1007252215954875No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGCT200.008488432527.0057895
CAGGCTA404.886074E-420.2543416
GATACCA1350.020.00428827
TGATACC1500.018.00385926
TTGATAC4450.017.90271427
CGTTGAT4400.017.18550125
GTTGATA4450.016.99240726
GCGTTGA4800.015.75337724
ATGGGGG550.004125545714.730435
TCCGCTA550.004125545714.730436
AGGCTAT600.0073476613.5028947
TATCAAC2050.013.1545181
TGCGTTG6200.012.19616323
GTATCAA1852.3137545E-911.66130351
CTGCGTT6900.011.15456522
GTGCTAT850.005542878511.120037
TCTGCGT11850.011.05300227
ATCAACG2552.5465852E-1110.5905052
TCAACGC2655.4569682E-1110.1908643
CTCTGCG13400.09.67371526
Produced by FastQC (version 0.11.2)