FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate1_15.3520000005b2f3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate1_15.3520000005b2f3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48914
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCTATGCCAACGTACTCTGCGTTGATACCAC3860.7891401234820297No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA2570.5254119474996933No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA2480.5070123073148792No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT2450.5008790939199411No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA2370.4845238582001063No Hit
CCCCTCAGCTATGCCAACGTACTCTGCGTTGAT1950.3986588706709735No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1910.39048125281105617No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA1890.38639244388109745No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA1710.34959316351146913No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA1330.2719057938422538No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1200.24532853579752217No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA1000.20444044649793516No Hit
CCCTTCAGCTATGCCAACGTACTCTGCGTTGAT920.18808521077810034No Hit
GTGAATGGGTCAGCTATGCCAAAAAAAAAAAAA880.17990759291818292No Hit
CCCCCTCAGCTATGCCAACGTACTCTGCGTTGA790.16150795273336876No Hit
CCCTCAGCTATGCCAACGTACTCTGCGTTGATA760.1553747393384307No Hit
CCTCAGCTATGCCAACGTACTCTGCGTTGATAC750.15333033487345138No Hit
CCCGTCAGCTATGCCAACGTACTCTGCGTTGAT700.1431083125485546No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA680.1390195036185959No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA670.13697509915361655No Hit
GCCCTCAGCTATGCCAACGTACTCTGCGTTGAT660.1349306946886372No Hit
CCTCTCAGCTATGCCAACGTACTCTGCGTTGAT650.13288629022365786No Hit
CCCCTTCAGCTATGCCAACGTACTCTGCGTTGA620.1267530768287198No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT580.1185754589688024No Hit
GAGTACGTTGGCAAAAAAAAAAAAAAAAAAAAA580.1185754589688024No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA520.10630903217892629No Hit
CCCATCAGCTATGCCAACGTACTCTGCGTTGAT490.10017581878398822No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTCG306.986933E-527.01946822
ACTCTTC306.986933E-527.01946821
TACTCTT306.986933E-527.01946820
TCTTCGT306.986933E-527.01946823
TTCGTTG307.035958E-526.99181225
TCGTTGA307.035958E-526.99181226
ATACCAC401.7968967E-523.64203327
CTTCGTT351.9906493E-423.15954424
GATACCA404.9434975E-420.24385826
TGATACC404.9434975E-420.24385825
CGTTGAT3300.016.7848227
TATCAAC2800.014.9418961
TCTGCGT12900.014.45227127
ATCAACG3050.013.717152
TCAACGC3200.013.0741593
TATCAGC956.8452726E-512.7855957
TTGATAC853.8417094E-412.71504427
CAACGCA3600.011.6214754
CTCTGCG16450.011.32179426
GTTGATA1257.150743E-510.80778727
Produced by FastQC (version 0.11.2)