FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate1_14.3520000005b2d9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate1_14.3520000005b2d9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47827
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCATACGTACTCAAAAAAAAAAAAAAAAAAA9281.940326593765028No Hit
CAACAGAGCATACGTACTCTGCGTTGATACCAC5281.1039789240387228No Hit
GAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2560.5352625086248354No Hit
GCATACGTACTCAAAAAAAAAAAAAAAAAAAAA2510.5248081627532565No Hit
GTGAATGGGCAACAGAGCAAAAAAAAAAAAAAA2450.5122629477073619No Hit
ACGCAGAGTGAATGGGCAACAGAGCAAAAAAAA2410.5038994710100989No Hit
TATCAACGCAGAGTGAATGGGCAACAGAGCATA2370.49553599431283585No Hit
GAGCATAAAAAAAAAAAAAAAAAAAAAAAAAAA1880.39308340477136344No Hit
ACGCAGAGTGAATGGGCAACAGAGAAAAAAAAA1860.38890166642273194No Hit
TATCAACGCAGAGTGAATGGGCAACAGAGCAAA1800.3763564513768373No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1720.35962949798231125No Hit
GTGAATGGGCAACAGAGAAAAAAAAAAAAAAAA1710.3575386288079955No Hit
TATCAACGCAGAGTGAATGGGCAACAGAGAAAA1440.3010851611014699No Hit
GTGAATGGGGCAACAGAGCAAAAAAAAAAAAAA1440.3010851611014699No Hit
GAGCATACGTACAAAAAAAAAAAAAAAAAAAAA1420.2969034227528383No Hit
GAGCATACGTAAAAAAAAAAAAAAAAAAAAAAA1410.2948125535785226No Hit
GTATCAACGCAGAGTGAATGGGCAACAGAGCAT1300.2718129926610492No Hit
CAACAGAGCAAAAAAAAAAAAAAAAAAAAAAAA1280.2676312543124177No Hit
ACGCAGAGTGAATGGGCAACAGAGCATACGTAC1250.26135864678947035No Hit
AACGCAGAGTGAATGGGCAACAGAGCAAAAAAA1190.2488134317435758No Hit
CCCCGCAACAGAGCATACGTACTCTGCGTTGAT1160.2425408242206285No Hit
CCGCAACAGAGCATACGTACTCTGCGTTGATAC1150.24044995504631272No Hit
ACGCAGAGTGAATGGGCAACAGAGCATAAAAAA1130.23626821669768122No Hit
GTATCAACGCAGAGTGAATGGGCAACAGAGCAA1080.22581387082610244No Hit
GTGAATGGGGCAACAGAGAAAAAAAAAAAAAAA1030.2153595249545236No Hit
GGGCAACAGAGCAAAAAAAAAAAAAAAAAAAAA1030.2153595249545236No Hit
GAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1010.21117778660589206No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA980.20490517908294476No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA960.20072344073431325No Hit
GTGAATGGGCAACAGAGCATAAAAAAAAAAAAA950.19863257155999747No Hit
GAGTGAATGGGCAACAGAGCAAAAAAAAAAAAA930.19445083321136597No Hit
GGTATCAACGCAGAGTGAATGGGCAACAGAGCA890.1860873565141029No Hit
CAACAGAGCATACGTACTCAAAAAAAAAAAAAA870.1819056181654714No Hit
GCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAA860.17981474899115563No Hit
GTGAATGGGGCAACAGAAAAAAAAAAAAAAAAA850.17772387981683985No Hit
GCAACAGAGCATACGTACTCTGCGTTGATACCA840.1756330106425241No Hit
GAGTGAATGGGCAACAGAGAAAAAAAAAAAAAA800.16726953394526103No Hit
GTGAATGGGCAACAGAGCATACGTACTCAAAAA790.16517866477094528No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA770.16099692642231375No Hit
AACGCAGAGTGAATGGGCAACAGAGAAAAAAAA760.158906057247998No Hit
GCGCAACAGAGCATACGTACTCTGCGTTGATAC750.15681518807368222No Hit
GTATCATCGCAGAGTGAATGGGCAACAGAGCAT710.14845171137641916No Hit
ATCAACGCAGAGTGAATGGGCAACAGAGCAAAA690.14426997302778766No Hit
GTGAATGGGCAACAGAAAAAAAAAAAAAAAAAA690.14426997302778766No Hit
GTATCAACGCAGAGTGAATGGGCAACAGAGAAA690.14426997302778766No Hit
GGGCAACAGAGCATACGTACTCTGCGTTGATAC670.14008823467915613No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA660.13799736550484035No Hit
TACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA650.1359064963305246No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA640.13381562715620884No Hit
AACGCAGAGTGAATGGGCAACAGAGCATACGTA600.12545215045894578No Hit
ACGCAGAGTGAATGGGCAACAGAAAAAAAAAAA600.12545215045894578No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT600.12545215045894578No Hit
GTATCAACGCAGAGTGAATGGGGCAACAGAGCA590.12336128128463003No Hit
CCCCCGCAACAGAGCATACGTACTCTGCGTTGA580.12127041211031425No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA570.11917954293599849No Hit
GGGCAACAGAGCATACGTACTCAAAAAAAAAAA560.11708867376168273No Hit
GCCCGCAACAGAGCATACGTACTCTGCGTTGAT560.11708867376168273No Hit
GGGCAACAGAGAAAAAAAAAAAAAAAAAAAAAA550.11499780458736697No Hit
CAACAGAGCATACGTACAAAAAAAAAAAAAAAA530.11081606623873544No Hit
GTATCATCGCAGAGTGAATGGGCAACAGAGCAA530.11081606623873544No Hit
GGGCAACAGAGCATAAAAAAAAAAAAAAAAAAA520.10872519706441967No Hit
GCATACGTACAAAAAAAAAAAAAAAAAAAAAAA520.10872519706441967No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA510.10663432789010392No Hit
CAACAGAGCATACGTACTCTGCGTTTATACCAC500.10454345871578816No Hit
ATGGGCAACAGAGCAAAAAAAAAAAAAAAAAAA490.10245258954147238No Hit
CAACAGAGCATACGTAAAAAAAAAAAAAAAAAA490.10245258954147238No Hit
GTGAATGGGGCAACAGAGCATAAAAAAAAAAAA490.10245258954147238No Hit
GAGTGAATGGGGCAACAGAGCAAAAAAAAAAAA490.10245258954147238No Hit
TATCAACGCAGAGTGAATGGGGCAACAGAGCAA480.10036172036715663No Hit
GAGCATACAAAAAAAAAAAAAAAAAAAAAAAAA480.10036172036715663No Hit
CAACAGAGCATAAAAAAAAAAAAAAAAAAAAAA480.10036172036715663No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACTCA257.7318447E-426.9916231
GTATCAT451.6460162E-623.9925541
TATCATC451.6460162E-623.9925542
ATCTACG352.0037236E-423.1356772
TCTACGC352.0037236E-423.1356773
TACGCAG352.0037236E-423.1356775
TATCTAC352.0037236E-423.1356771
TCCTGCA300.002237245422.493025
TCGCAGA559.37961E-619.6302727
ATCATCG559.37961E-619.6302723
ATCGCAG559.37961E-619.6302726
TCATCGC559.37961E-619.6302724
CGCTGAG350.005466966419.2797322
CTGAGTG350.005466966419.2797324
ACGCTGA350.005466966419.2797321
GCTGAGT350.005466966419.2797323
TGAGTGA350.005466966419.2797325
CATCGCA601.9860408E-517.9944155
CTACGCA500.002210034816.1949754
ATACCAC707.379637E-515.43995227
Produced by FastQC (version 0.11.2)