FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate1_1.3520000005b1e7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate1_1.3520000005b1e7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51852
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCAGAGTGAATGGGCAGACACAAAAAAAAAA9771.8842089022602795No Hit
GTGAATGGGCAGACACAAAAAAAAAAAAAAAAA9631.8572089794029158No Hit
CACACGTACTCAAAAAAAAAAAAAAAAAAAAAA8161.5737097894006018No Hit
TATCAACGCAGAGTGAATGGGCAGACACAAAAA7351.4174959500115714No Hit
CACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA6321.218853660418113No Hit
GAGTGAATGGGCAGACACAAAAAAAAAAAAAAA4970.958497261436396No Hit
AACGCAGAGTGAATGGGCAGACACAAAAAAAAA4580.8832831906194555No Hit
GTGAATGGGCAGACAAAAAAAAAAAAAAAAAAA4520.8717117951091569No Hit
ACGCAGAGTGAATGGGCAGACAAAAAAAAAAAA3960.7637121036797038No Hit
GTATCAACGCAGAGTGAATGGGCAGACACAAAA3830.7386407467407237No Hit
GACACAAAAAAAAAAAAAAAAAAAAAAAAAAAA3800.7328550489855743No Hit
GAGCAGACACACGTACTCTGCGTTGATACCACT3540.682712335107614No Hit
CACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3260.6287124893928875No Hit
TATCAACGCAGAGTGAATGGGCAGACAAAAAAA3120.6017125665355242No Hit
TATCAACGCAGAGTGAATGGGCAGACACACGTA3020.5824269073516933No Hit
GACACACGTACTCAAAAAAAAAAAAAAAAAAAA2400.46285582041194173No Hit
ATCAACGCAGAGTGAATGGGCAGACACAAAAAA2390.46092725449355854No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2230.4300701997994291No Hit
GTATCATCGCAGAGTGAATGGGCAGACACAAAA2180.42042737020751375No Hit
ACGCAGAGTGAATGGGCAGACACACGTACTCAA2100.40499884286044896No Hit
CACGTACAAAAAAAAAAAAAAAAAAAAAAAAAA2070.39921314510529976No Hit
GTGAATGGGCAGACACACGTACTCAAAAAAAAA2070.39921314510529976No Hit
CACGTAAAAAAAAAAAAAAAAAAAAAAAAAAAA2070.39921314510529976No Hit
GACACACAAAAAAAAAAAAAAAAAAAAAAAAAA2050.3953560132685335No Hit
GAGTGAATGGGCAGACAAAAAAAAAAAAAAAAA2030.39149888143176736No Hit
AACGCAGAGTGAATGGGCAGACAAAAAAAAAAA2000.3857131836766181No Hit
GTATCAACGCAGAGTGAATGGGCAGACACACGT1780.3432847334721901No Hit
GTGAATGGGCAGACACACAAAAAAAAAAAAAAA1760.3394276016354239No Hit
GTATCAACGCAGAGTGAATGGGCAGACAAAAAA1750.3374990357170408No Hit
TATCTACGCAGAGTGAATGGGCAGACACAAAAA1680.32399907428835917No Hit
CAACGCAGAGTGAATGGGCAGACACAAAAAAAA1640.31628481061482683No Hit
CGTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA1630.31435624469644374No Hit
CACACAAAAAAAAAAAAAAAAAAAAAAAAAAAA1610.3104991128596775No Hit
ACGCAGAGTGAATGGGCAGACACACAAAAAAAA1520.2931420195942297No Hit
GACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1430.27578492632878193No Hit
CAACTCAGAGTGAATGGGCAGACACAAAAAAAA1390.26807066265524954No Hit
ACGCTGAGTGAATGGGCAGACACAAAAAAAAAA1350.2603563989817172No Hit
GGCAGACACAAAAAAAAAAAAAAAAAAAAAAAA1330.256499267144951No Hit
AACGCAGAGTGAATGGGCAGACACACGTACTCA1240.2391421738795032No Hit
GTATCATCGCAGAGTGAATGGGCAGACACACGT1200.23142791020597087No Hit
AACGTAGAGTGAATGGGCAGACACAAAAAAAAA1190.22949934428758775No Hit
GAGTGAATGGGCAGACACACGTACTCAAAAAAA1140.21985651469567227No Hit
ATCAACGCAGAGTGAATGGGCAGACACACGTAC1030.19864228959345828No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1000.19285659183830905No Hit
CAACTCAGAGTGAATGGGCAGACAAAAAAAAAA980.18899946000154286No Hit
ATCAACGCAGAGTGAATGGGCAGACAAAAAAAA980.18899946000154286No Hit
TATCAACGCAGAGTGAATGGGCAGACACACAAA970.18707089408315974No Hit
GTATCATCGCAGAGTGAATGGGCAGACAAAAAA960.1851423281647767No Hit
GTGAATGGGCAGACACACGAAAAAAAAAAAAAA950.18321376224639357No Hit
ACGCAGAGTGAATGGGCAGACACACGAAAAAAA930.1793566304096274No Hit
TATCAACGCAGAGTGAATGGGCAGACACACGAA920.17742806449124432No Hit
GGTATCAACGCAGAGTGAATGGGCAGACACAAA910.1754994985728612No Hit
CACACGAAAAAAAAAAAAAAAAAAAAAAAAAAA910.1754994985728612No Hit
CACACGTACTCTAAAAAAAAAAAAAAAAAAAAA850.1639281030625627No Hit
TATCTACGCAGAGTGAATGGGCAGACAAAAAAA840.16199953714417958No Hit
CACGTACTCTAAAAAAAAAAAAAAAAAAAAAAA830.1600709712257965No Hit
GAGTGAATGGGCAGACACACAAAAAAAAAAAAA830.1600709712257965No Hit
TCAACGCAGAGTGAATGGGCAGACACAAAAAAA830.1600709712257965No Hit
TATCTACGCAGAGTGAATGGGCAGACACACGTA800.15428527347064722No Hit
AACGCAGAGTGAATGGGCAGACACACAAAAAAA740.14271387796034868No Hit
CCCATGAGCAGACACACGTACTCTGCGTTGATA720.1388567461235825No Hit
CACACGTAAAAAAAAAAAAAAAAAAAAAAAAAA720.1388567461235825No Hit
GAATGGGCAGACACAAAAAAAAAAAAAAAAAAA700.13499961428681634No Hit
CAACGCAGAGTGAATGGGCAGACAAAAAAAAAA610.1176425210213685No Hit
CACACGTACAAAAAAAAAAAAAAAAAAAAAAAA600.11571395510298543No Hit
ACGCAGAGTGAATGGGCAAAAAAAAAAAAAAAA590.11378538918460233No Hit
GTGAATGGGCAGACACACGTAAAAAAAAAAAAA560.10799969142945307No Hit
GTATCAACGCAGAGTGAATGGGCAGACACACAA560.10799969142945307No Hit
CACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA550.10607112551106997No Hit
GGTATCAACGCAGAGTGAATGGGCAGACACACG550.10607112551106997No Hit
ATCATCGCAGAGTGAATGGGCAGACACAAAAAA550.10607112551106997No Hit
GAGTAAATGGGCAGACACAAAAAAAAAAAAAAA550.10607112551106997No Hit
ATCAACGCAGAGTGAATGGGCAGACACACAAAA540.10414255959268687No Hit
AACGCAGAGTGAATGGGCAGACACACGAAAAAA530.10221399367430378No Hit
ACGCAGAGTGAATGGGCAGACACACGTAAAAAA520.1002854277559207No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGAG257.728481E-426.9990352
CTGAGTG257.728481E-426.9990354
CAACTCA700.026.9990351
GTATCAT850.026.9990351
GTATTAA200.00853757326.9990351
GCTGAGT200.00853757326.9990353
TGAGTGA257.728481E-426.9990355
TATCATC900.025.4990862
AACGTAG551.38152245E-824.5445771
AACTCAG752.7284841E-1123.3991622
GACATGA352.0032361E-423.142036
ACGCTGA300.00223625722.4991971
ACTCAGA806.548362E-1121.9367163
CTCAGAG806.548362E-1121.9367164
ATCTACG757.039489E-1021.5992282
TCTACGC757.039489E-1021.5992283
TATCTAC757.039489E-1021.5992281
ATCATCG1000.021.5992283
ATCGCAG1000.021.5992286
TCATCGC1000.021.5992284
Produced by FastQC (version 0.11.2)