FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate8_94.351000000610f7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate8_94.351000000610f7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1250
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTGCAAGGACTGTCTCTTATACACA70.5599999999999999No Hit
TACTGCAAGGAGGCTGTCTCTTATACA30.24No Hit
TACTGCAAGGAGCCAGTGCATTTTTTT20.16No Hit
TACTGCAAGGAGCGTAACGGTTTTTTT20.16No Hit
TACTGCAAGGAGGGAGAGCATTTTTTT20.16No Hit
TACTGCAAGGAGGCCGGGTGTTTTTTT20.16No Hit
TACTGCAAGGAGCGGTTAGGTTTTTTT20.16No Hit
TACTGCAAGGAGTTGTTCTATTTTTTT20.16No Hit
TACTGCAAGGAGCCAGGTCATTTTTTT20.16No Hit
TACTGCAAGGAGGCTATGTGTCTGTCT20.16No Hit
TACTGCAAGGAGGGGGGTCGTTTTTTT20.16No Hit
TACTGCAAGGAGTGTGGGGGTTTTTTT20.16No Hit
TACTGCAAGGAGGAGGGGCACTGTCTC20.16No Hit
TACTGCAAGGAGTCGGTATATCTGTCT20.16No Hit
TACTGCAAGGAGAGCTGGGGTTTTTTT20.16No Hit
TACTGCAAGGAGGTTTGTGGTTTTTTT20.16No Hit
TACTGCAAGGAGATCATTTGTTTTTTT20.16No Hit
TACTGCAAGGAGGGGTTCCATTTTTTT20.16No Hit
TACTGCAAGGAGTAGTGCTCTTTTTTT20.16No Hit
TACTGCAAGGAGATCGTAAGTTTTTTT20.16No Hit
TACTGCAAGGAGCGGGGCGGTTTTTTT20.16No Hit
TACTGCAAGGAGATTAGCTATTTTTTT20.16No Hit
TACTGCAAGGAGGGGGGCGGTTTTTTT20.16No Hit
TACTGCAAGGAGGACGGGAGTTTTTTT20.16No Hit
TACTGCAAGGAGGCTCTTGGCTGTCTC20.16No Hit
TACTGCAAGGAGGCGCGGGCTCTGTCT20.16No Hit
TACTGCAAGGAGGTCTAGCCTTTTTTT20.16No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA1150.021.752
TACTGCA1150.021.751
CTGCAAG1150.021.753
GCAAGGA1250.020.8800015
TGCAAGG1250.020.8800014
GTTTTTT250.001916530120.88000120
AAGGAGT351.5117206E-520.8800017
AAGGAGG507.2686817E-920.8800017
CAAGGAG1200.020.8800016
TTTTTTT555.0567905E-1020.8821
GCTGTCT404.272736E-518.2720
CTGTCTC508.80972E-616.70421
Produced by FastQC (version 0.11.2)