FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate8_8.3510000006062d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate8_8.3510000006062d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23158
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGTGCTCATGCTGTCTCTTATACAC4391.8956732014854478No Hit
ACAGTGCTCATGGGCTGTCTCTTATAC1350.5829518956732015No Hit
ACAGTGCTCATGCTGTCTCTTCTACAC1240.535452111581311No Hit
ACAGTGCTCATGGCTGTCTCTTATACA500.21590810950859315No Hit
ACAGTGCTCATGGCCTGTCTCTTATAC450.19431729855773383No Hit
ACAGTGCTCATGAGCTGTCTCTTATAC390.16840832541670264No Hit
ACAGTGCTCATGGTCTGTCTCTTATAC360.15545383884618705No Hit
ACAGTGCTCATGGGGGGCTGTCTCTTA280.12090854132481216No Hit
ACAGTGCTCATGGGGGGGCTGTCTCTT280.12090854132481216No Hit
ACAGTGCTCATGACCTGTCTCTTATAC250.10795405475429658No Hit
ACCGTGCTCATGCTGTCTCTTCTACAC240.1036358925641247No Hit
ACAGTGCTCCTGTCTCTTATACACATC240.1036358925641247No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTGC303.3664197E-421.3568291
AGTGCTC20900.021.3568293
CTGTGCT303.3664197E-421.3568292
CCGTGCT1250.021.3568272
ACCGTGC1250.021.3568271
CGTGCTC1250.021.3568273
ACAGTGC20800.021.3054891
CAGTGCT20900.021.2035482
TCATGGC2550.020.9416878
TGGTTTT651.10958354E-1020.94168518
ATGGGGT354.4521446E-520.94168510
ATGGGGG751.8189894E-1220.94168510
ATGGGGA354.4521446E-520.94168510
CATGATG456.006903E-720.9416859
ATGGGAG354.4521446E-520.94168510
CATGAGT250.003335434520.9416859
CATGAGG303.8470683E-420.9416859
CATGAGC354.4521446E-520.9416859
CATGAGA456.006903E-720.9416859
CATGAAG456.006903E-720.9416859
Produced by FastQC (version 0.11.2)