FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate8_75.35100000060e9f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate8_75.35100000060e9f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1250
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGACCAACGAGGGCGACGTTTTTTT20.16No Hit
TTCGACCAACGAAGGGCCTGTTTTTTT20.16No Hit
TTCGACCAACGAGGCGGAGGTTTTTTT20.16No Hit
TTCGACCAACGAGCTCTTCGTTTTTTT20.16No Hit
TTCGACCAACGATTTTAGAATTTTTTT20.16No Hit
TTCGACCAACGAACATGATGTTTTTTT20.16No Hit
TTCGACCAACGATGGATGGGTTTTTTT20.16No Hit
TTCGACCAACGACGAGCTGATTTTTTT20.16No Hit
TTCGACCAACGACAAGGACGTTTTTTT20.16No Hit
TTCGACCAACGACCGGCGCGTTTTTTT20.16No Hit
TTCGACCAACGAAGGAGGAGTTCTGTC20.16No Hit
TTCGACCAACGATTAAAGAGTTTTTTT20.16No Hit
TTCGACCAACGAGTCACAGCTTTTTTT20.16No Hit
TTCGACCAACGAGGCTCTTGTTTTTTT20.16No Hit
TTCGACCAACGAGCGCCTGGTTTTTTT20.16No Hit
TTCGACCAACGAAGGCGGGGTTTTTTT20.16No Hit
TTCGACCAACGACAAAAGCGTTTTTTT20.16No Hit
TTCGACCAACGAGTGTTGGGTTTTTTT20.16No Hit
TTCGACCAACGAAGTAGCTATTTTTTT20.16No Hit
TTCGACCAACGAGGAGTGGGTTTTTTT20.16No Hit
TTCGACCAACGACTGGCGGGTTTTTTT20.16No Hit
TTCGACCAACGAAAGGGGTGTTTTTTT20.16No Hit
TTCGACCAACGAGTGGCGACTTTTTTT20.16No Hit
TTCGACCAACGATGGTAGCTGTCTCTT20.16No Hit
TTCGACCAACGACGCGTGTGTTTTTTT20.16No Hit
TTCGACCAACGAAAGGTCCCTTTTTTT20.16No Hit
TTCGACCAACGACTGTCTCTTCTACAC20.16No Hit
TTCGACCAACGCTGTCTCTTATACACA20.16No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCA1050.021.752
TTCGACC1050.021.751
CGACCAA1100.021.753
CCAACGA1200.020.8800016
TTTTTTT800.020.88000121
GTTTTTT301.7407999E-420.88000120
ACCAACG1250.020.8800015
GACCAAC1250.020.8800014
CAACGAT351.5117206E-520.8800017
CAACGAG250.001916530120.8800017
CAACGAC301.7407999E-420.8800017
CAACGAA301.7407999E-420.8800017
CTTTTTT301.7407999E-420.88000120
Produced by FastQC (version 0.11.2)