FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate8_7.35100000060603.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate8_7.35100000060603.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28621
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCAGCAATGCCTGTCTCTTATACAC1350.4716816323678418No Hit
CTTCAGCAATGCCTGTCTCTTCTACAC370.12927570664896404No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCAGC23850.021.255321
TTCCGCA2100.021.255322
GCAGCAA557.034032E-921.255323
CTGCAGC557.034032E-921.255321
TTCAGCA23900.021.255322
TGCAGCA557.034032E-921.255322
CTTCCGC2100.021.255321
CGTCAGC354.0100684E-521.2553181
CTCCAGC506.096343E-821.2553181
TCCAGCA506.096343E-821.2553182
TCAGCAA24500.021.2553183
GTCAGCA354.0100684E-521.2553182
TCCTTTT250.003339427520.95804218
GCGGGCC250.003339427520.95804211
GCCTTTT507.084418E-820.95804218
CAATGCG12050.020.9580427
CAATGCC5750.020.9580427
CAATGCA5500.020.9580427
CAGTTTT456.065293E-720.95804218
CAGCAAT25900.020.9580424
Produced by FastQC (version 0.11.2)