FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate8_55.35100000060c1a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate8_55.35100000060c1a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1250
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCAGCAATGCCTGTCTCTTATACAC30.24No Hit
CTTCAGCAATGCAGTCGGGGTTTTTTT30.24No Hit
CTTCAGCAATGCGGATTACATTTTTTT20.16No Hit
CTTCAGCAATGCACCTGTCTCTTATAC20.16No Hit
CTTCAGCAATGCGTAGGCTGTCTCTTA20.16No Hit
CTTCAGCAATGCCGCATCGGTTTTTTT20.16No Hit
CTTCAGCAATGCGCGCAGCGTTTTTTT20.16No Hit
CTTCAGCAATGCGGAGGCAGTTTTTTT20.16No Hit
CTTCAGCAATGCATAAATGATTTTTTT20.16No Hit
CTTCAGCAATGCTAGCTGTGTTTTTTT20.16No Hit
CTTCAGCAATGCCGTTGATCCTGTCTC20.16No Hit
CTTCAGCAATGCGCTGCCACTTTTTTT20.16No Hit
CTTCAGCAATGCAGATGGAGTTTTTTT20.16No Hit
CTTCAGCAATGCACTAGCGCTTTTTTT20.16No Hit
CTTCAGCAATGCACCAGAGCTTTTTTT20.16No Hit
CTTCAGCAATGCCGGAGGAGTTTTTTT20.16No Hit
CTTCAGCAATGCGGCAGGCGTTTTTTT20.16No Hit
CTTCAGCAATGCGGGGAGCGTTTTTTT20.16No Hit
CTTCAGCAATGCCATATAGCTTTTTTT20.16No Hit
CTTCAGCAATGCAGGGCCTGTCTCTTA20.16No Hit
CTTCAGCAATGCGGTTTGAGTTTTTTT20.16No Hit
CTTCAGCAATGCAATGTGAGTTTTTTT20.16No Hit
CTTCAGCAATGCCTGTCTCTTCTACAC20.16No Hit
CTTCAGCAATGCGGAGGTTATTTTTTT20.16No Hit
CTTCAGCAATGCTTTGTTGCTTTTTTT20.16No Hit
CTTCAGCAATGCAGCGGGCATTTTTTT20.16No Hit
CTTCAGCAATGCGGCGGGGCTTTTTTT20.16No Hit
CTTCAGCAATGCATTTCTTATTTTTTT20.16No Hit
CTTCAGCAATGCCGTTCGACTTTTTTT20.16No Hit
CTTCAGCAATGCCTGGGGTCTTTTTTT20.16No Hit
CTTCAGCAATGCCGCACGCCTTTTTTT20.16No Hit
CTTCAGCAATGCTTGGGTGCTTTTTTT20.16No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGCG459.277028E-821.0000027
CAATGCA301.6740938E-421.0000027
GCAATGC1200.021.0000026
CTTTTTT301.6740938E-421.00000220
CTTCAGC1050.021.01
CAGCAAT1050.021.04
TTTTTTT950.021.021
GTTTTTT351.44566875E-521.020
AGCAATG1050.021.05
TTCAGCA1050.021.02
ATTTTTT351.44566875E-521.020
CAATGCT250.001853412620.9999987
TCAGCAA1000.020.9999983
Produced by FastQC (version 0.11.2)