FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate8_47.35100000060b0e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate8_47.35100000060b0e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35859
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCAGACTGGAGGCTGTCTCTTATAC630.17568811177110347No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGA1900.021.4108431
TAGCAGA31750.021.4108431
ATCAGAC505.7443685E-821.4108432
AGCAGAC31800.021.4108432
TCAGACT505.7443685E-821.4108433
GCAGACT32000.021.4108433
AGCCGAC1900.021.4108432
GCCGACT1900.021.4108433
GGATGTA250.003379153820.93305610
GGAGAAT250.003379153820.93305610
GGATGGA250.003379153820.93305610
ATCTTTT250.003379153820.93305618
GAGTGAG250.003379153820.93305611
GGACTTT250.003379153820.93305617
GGTCTTT250.003379153820.93305617
TTATTTT507.304516E-820.93305618
TTTTCTG250.003379153820.93305621
GTATTTT558.583811E-920.93305618
GGATTGT250.003379153820.93305610
CATTTTT1100.020.93305619
Produced by FastQC (version 0.11.2)