FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate8_46.35100000060ae3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate8_46.35100000060ae3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70469
Sequences flagged as poor quality0
Sequence length27
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTGCAAGGACTGTCTCTTATACACA2180.30935588698576677No Hit
TACTGCAAGGAGGCTGTCTCTTATACA730.10359165022917878No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACAAG1300.021.4585173
ACTGCAA64100.021.4585172
TACTACA1300.021.4585171
ACTACAA1300.021.4585172
TTGCAAG1050.021.4585153
TTCTGCA900.021.4585151
CTCCAAG455.042948E-721.4585153
ACTCCAA455.042948E-721.4585152
TAATGCA701.0913936E-1121.4585151
TATTGCA1050.021.4585151
TCTGCAA900.021.4585152
ATTGCAA1050.021.4585152
ATGCAAG701.0913936E-1121.4585153
AATGCAA701.0913936E-1121.4585152
CTGCAAG64950.021.4585153
TACTCCA455.042948E-721.4585151
TACTGCA64150.021.441791
TGGTTTT850.020.9254818
GAGGCGT558.987627E-920.9254810
GAGGTGA354.650045E-520.9254810
Produced by FastQC (version 0.11.2)