FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate8_45.35100000060ac9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate8_45.35100000060ac9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9671
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGACGCTGTCTCTTATACACATC130.13442250025850483No Hit
ATCTCGACGCTAGGGCTGTCTCTTATA110.11374211560335024No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTAGA850.021.3915331
CTCGACG8500.021.2789483
TCTAGAC850.021.2789482
ATCTCGA8400.021.2642041
TCTCGAC8450.021.1530362
GGTTTTT1250.020.94818919
TGGTTTT250.003191504220.94818718
GTGTTTT303.6180057E-420.94818718
GGGTTTT607.585186E-1020.94818718
ACGCTAT1750.020.9481877
ACGCTAC2450.020.9481877
ACGCTAA2000.020.9481877
TCGACGC8800.020.9481874
GCTAGTG250.003191504220.9481879
GCTAGGG505.962647E-820.9481879
GCTAGCG250.003191504220.9481879
GCTAGAG455.2711766E-720.9481879
GTTCTGT250.003191504220.94818720
GGTCTGT354.1034582E-520.94818719
TTTTTTT5550.020.94818721
Produced by FastQC (version 0.11.2)