FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate8_44.35100000060aa0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate8_44.35100000060aa0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34939
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCACT701.0913936E-1121.2351253
TTTGCCA701.0913936E-1121.2351251
TTGCCAC701.0913936E-1121.2351252
TTAGACA950.021.2351231
TTCGCCA1100.021.2351231
TAAGCCA250.003107292821.2351231
TAGCCAC30500.021.2351232
CGCCACT1100.021.2351233
GAGCCAC557.2523108E-921.2351232
AAGCCAC250.003107292821.2351232
TTAGCCA30450.021.2351231
TGAGCCA557.2523108E-921.2351231
AGCCACT31500.021.2014183
CTCTGGA2100.020.9613198
TTGTTTT751.8189894E-1220.96131918
CTCTGCT1600.020.9613198
CTCTGCG2000.020.9613198
CTCTGCA1350.020.9613198
CTCTGAG2400.020.9613198
TCCTTTT303.8772466E-420.96131918
Produced by FastQC (version 0.11.2)