FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate8_43.35100000060a86.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate8_43.35100000060a86.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25659
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGCTGGATGCTGTCTCTTATACACA3271.274406640944698No Hit
CTAGCTGGATGTCTGTCTCTTATACAC910.3546513893760474No Hit
CTAGCTGGATGTGGGGGCTGTCTCTTA450.175377060680463No Hit
CTAGCTGGATCTGTCTCTTATACACAT410.1597879886199774No Hit
CTAGCTGGATGTGGGGCTGTCTCTTAT330.1286098444990062No Hit
CTAGCTGGATGTGGGCTGTCTCTTATA320.1247125764838848No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTGGA303.5805057E-421.1771663
AGATGGA659.640644E-1121.1771663
CTAGATG659.640644E-1121.1771661
CTCGCTG701.0913936E-1121.1771661
AGCTGGA23700.021.1771663
TAGATGG659.640644E-1121.1771662
TTGCTGG303.5805057E-421.1771662
CTAGCTG23400.021.1771641
TAGCTGG23450.021.1320112
TTGTTTT558.127245E-920.9707618
GGATGTG10900.020.970767
TGTTGGG609.495125E-1020.9707610
TGTTGCG303.8268432E-420.9707610
GGGTCTG354.4289263E-520.9707618
GGATGCT506.967275E-820.970767
GGGTTTT1300.020.9707618
GCCTTTT405.140493E-620.9707618
CAGTTTT558.127245E-920.9707618
TATACAC455.9796366E-720.9707620
ACGTTTT354.4289263E-520.9707618
Produced by FastQC (version 0.11.2)