FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate8_42.35100000060a6c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate8_42.35100000060a6c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22434
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCAGA18850.021.322731
CAAGAGA250.002999465221.3227273
TCAAGAG250.002999465221.3227272
ACAGAGA404.402087E-621.3227273
TACAGAG404.402087E-621.3227272
TTCAAGA250.002999465221.3227271
TTACAGA404.402087E-621.3227271
CCAGAGA19450.021.3227273
TTTCAGA404.402087E-621.3227271
TCCAGAG18900.021.3227272
GCCAGAG250.002999465221.3227272
TGCCAGA250.002999465221.3227271
TCAGAGA404.402087E-621.3227273
TTCAGAG404.402087E-621.3227272
TTCCCGA1400.021.3227251
CCCGAGA1400.021.3227253
TCCCGAG1400.021.3227252
TTTCTGT250.003288694720.98881321
TTCTGTC506.803384E-820.98881321
GGTTTTT1450.020.94196519
Produced by FastQC (version 0.11.2)