FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate8_40.35100000060a29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate8_40.35100000060a29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29146
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAGCTTGACCTGTCTCTTATACAC1360.4666163452960955No Hit
GTTGAGCTTGACCTGTCTCTTCTACAC370.12694709394084952No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGAGC23850.021.346761
GGTGAGC250.003001648521.346761
TGCGCTT2800.021.346763
TTGAGCT23850.021.346762
TGGAGCT404.435993E-621.346762
GATGAGC250.003001648521.346761
TTGCGCT2800.021.346762
GTTGCGC2800.021.346761
ATGAGCT250.003001648521.346762
TGAGCTT24500.021.3031943
GAGCTTG25850.020.94334
TTGACTG2250.020.94338
GCTTGAC28900.020.94336
CTTGACT5300.020.94337
CTTGACC5100.020.94337
AGGGGTT303.878255E-420.94329816
TTGTTTT651.1459633E-1020.94329818
GAGCGTT250.003354480320.94329816
GTGTTTT1450.020.94329818
GCCTTTT456.1141145E-720.94329818
Produced by FastQC (version 0.11.2)