FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate8_4.351000000605a5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate8_4.351000000605a5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11184
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGACGTCGATACCTGTCTCTTATACAC1651.4753218884120172No Hit
AGACGTCGATACCTGTCTCTTCTACAC600.5364806866952789No Hit
AGACGTCGATACGGCTGTCTCTTATAC150.13412017167381973No Hit
AGACGTCGATACGGGGGGGGTTTTTTT130.11623748211731044No Hit
AGACGTCGATACACCTGTCTCTTATAC130.11623748211731044No Hit
AGACGTCGATACCCTGTCTCTTATACA120.1072961373390558No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTCG353.411297E-521.4976982
GACGTCG9250.021.4976982
AGTCGTC353.411297E-521.4976981
TCGTCGA353.411297E-521.4976983
GCCGTCG1000.021.4976982
AGCCGTC1000.021.4976981
ACGTCGA9400.021.4976943
AGACGTC9300.021.3821161
TCGATAC11100.020.9192856
CAGTTTT250.003248874620.91928318
CGATACG4250.020.9192837
CGATACC2450.020.9192837
CGATACA1700.020.9192837
AAATTTT250.003248874620.91928318
GATACTT800.020.9192838
GATACTG1150.020.9192838
GATACTC303.702499E-420.9192838
GATACTA455.502716E-720.9192838
AATTTTT506.274604E-820.91928319
GATACGC800.020.9192838
Produced by FastQC (version 0.11.2)