FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate8_39.35100000060a1c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate8_39.35100000060a1c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9672
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTCGTCAGCTGTCTCTTATACACAT230.23779983457402812No Hit
ACTTCGTCAGCCTGTCTCTTATACACA210.21712158808933005No Hit
ACTTCGTCAGCTCCTGTCTCTTATACA150.15508684863523575No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTCA8050.021.1884823
CTTAGTC800.021.1884822
ACTTAGT800.021.1884821
TTAGTCA800.021.1884823
ACTTCGT7900.021.188481
CTTCGTC7900.021.188482
CAGCTCG658.367351E-1120.9689128
CAGCTCC250.003173243720.9689128
CAGCTCA455.221209E-720.9689128
CAGCTAT800.020.9689128
CAGCTAG1000.020.9689128
CAGCTAC303.593905E-420.9689128
CAGCTAA505.900438E-820.9689128
TGGTTTT354.072267E-520.96891218
CGTCAGC8650.020.9689125
GGGTTTT455.221209E-720.96891218
AGCTCTG354.072267E-520.9689129
AGCTCGG354.072267E-520.9689129
AGCTTGG303.593905E-420.9689129
AGCTTAA250.003173243720.9689129
Produced by FastQC (version 0.11.2)