FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate8_37.351000000609d7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate8_37.351000000609d7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23029
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAGTACCGCTGTCTCTTATACACA1220.5297668157540492No Hit
CTCTAGTACCGAGGCTGTCTCTTATAC420.18237873984975464No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGT250.00300537521.3185841
CTTTAGT303.4067986E-421.3185841
CTCTCGT1050.021.3185841
ATAGTAC250.00300537521.3185843
TTTAGTA303.4067986E-421.3185842
CTAGTAC20750.021.3185843
CCTAGTA404.418429E-621.3185842
TATAGTA250.00300537521.3185842
TTAGTAC303.4067986E-421.3185843
CCCTAGT404.418429E-621.3185841
TCTAGTA20200.021.3185832
CTCTAGT20200.021.3185831
GTACCGC558.11815E-920.9478286
TGTTTTT2200.020.94782819
GTATTTT558.11815E-920.94782818
CCGAGGC558.11815E-920.9478289
CCGAGGA558.11815E-920.9478289
CCGAGCA558.11815E-920.9478289
CTGTTTT558.11815E-920.94782818
CCGATGT558.11815E-920.9478289
Produced by FastQC (version 0.11.2)