FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate8_35.35100000060994.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate8_35.35100000060994.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41781
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAGAAGGACCTGTCTCTTATACACA3880.9286517795170054No Hit
CACAGAAGGACTGGGGGCTGTCTCTTA760.18190086402910413No Hit
CACAGAAGGACTGGGGCTGTCTCTTAT430.10291759412172996No Hit
CACAGAAGGACTGCTGTCTCTTATACA430.10291759412172996No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGAAG37150.021.1741242
CTCAGAA800.021.1741241
AAGAAGG250.00317021921.1741243
CAAAGAA250.00317021921.1741241
CAGAAGG37900.021.1741243
CACAGAA37150.021.1741241
AAAGAAG250.00317021921.1741242
CCGAAGG2100.021.1741243
ATAGAAG701.2732926E-1121.1741222
TTGTTTT507.237213E-820.97125818
TGGTTTT800.020.97125818
ACTGCGC354.515176E-520.97125810
ACTGCGA250.003352772420.97125810
ACTGTGG456.168357E-720.97125810
ACTGCCG405.2697196E-620.97125810
ACTGTAT250.003352772420.97125810
GTGTTTT651.1823431E-1020.97125818
GGGTTTT1000.020.97125818
CAGTTTT558.507413E-920.97125818
GGCATTT507.237213E-820.97125817
Produced by FastQC (version 0.11.2)