FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate8_31.3510000006091d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate8_31.3510000006091d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29215
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATCG455.627444E-721.124143
CATACAT455.627444E-721.124141
CAGCCAT950.021.1241381
AGCCATC950.021.1241382
GAAATCG1100.021.1241383
TGACATC250.003191068821.1241382
CTGACAT250.003191068821.1241381
GACATCG26100.021.1241383
GCCATCG950.021.1241383
CAGAAAT1100.021.1241381
CAGACAT25950.021.1241361
AGACATC25950.021.1241362
CGCAAGC1950.020.9794548
TTGTTTT751.8189894E-1220.97945218
CAAGAGG250.00332137220.97945210
CAATGGT250.00332137220.97945210
CAATGGG507.021117E-820.97945210
GCCTTTT303.833781E-420.97945218
CAGTTTT303.833781E-420.97945218
CAATGAG250.00332137220.97945210
Produced by FastQC (version 0.11.2)