FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate8_30.351000000608f2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate8_30.351000000608f2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12764
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTGTGACGAACCTGTCTCTTATACAC810.6345973049200877No Hit
AGTGTGACGAACCTGTCTCTTCTACAC230.18019429645879034No Hit
AGTGTGACGAACGGGGGGGGTTTTTTT160.12535255405828893No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGACG403.8486505E-621.5080663
GAGTGAC250.002783106221.5080662
AGGGTGA403.8486505E-621.5080661
AGAGTGA250.002783106221.5080661
AGTGACG250.002783106221.5080663
GTGTGAC11350.021.5080662
GGGTGAC403.8486505E-621.5080662
TGTGACG11400.021.5080643
AGTGTGA11400.021.413731
AACGCGC250.003276115320.91764810
GTGTTTT354.2898406E-520.91764818
GGGTTTT701.0913936E-1120.91764818
AACGTCG250.003276115320.91764810
AACCGGG354.2898406E-520.91764810
GAACGTT354.2898406E-520.9176489
GAACGTG506.4661435E-820.9176489
GTATTTT250.003276115320.91764818
GAACGGT354.2898406E-520.9176489
GAACGCG557.408744E-920.9176489
GAACGCC354.2898406E-520.9176489
Produced by FastQC (version 0.11.2)